| 货号 | ACC-052-50ul |
| 描述 | Each antibody ordered from Alomone Labs is supplied with its corresponding control peptide (antigen), free of charge. A Rabbit Polyclonal Antibody to TRPP1 (PKD2) Channel |
| 反应种属 | H, M, R |
| 应用 | IC, IH, WB |
| 供应商 | Alomone |
| 背景 | Transient receptor potential (TRP) channels are relatively non-selective ion channels enabling the exchange of cations down their electrochemical gradient. This exchange enables the intracellular rise in Na+ and Ca2+ concentration and ultimately in the cell membrane depolarization, important for action potential propagation and muscle contraction1. They are activated by an extremely broad range of stimuli namely, temperature, voltage, pH, endocrine factors as well as signaling molecules2. The TRP channel family is composed of 28 members divided in 7 subgroups: TRPV,TRPC,TRPM,TRPA, TRPN, TRPPandTRPML. All members of the TRP family have 6 transmembrane (TM) domains, and a pore domain between the fifth (S5) and sixth (S6) transmembrane domains. In general, TRP channels enable the passage of either Na+ or Ca2+ ions with little or no preference. However, some channels do exhibit some selectivity. Also, TRP channels do not display the positive charges in the S4 voltage-sensing domain like most voltage sensitive channels, although they do display voltage dependency3. In addition, TRP channels have in the C-terminal intracellular region to the S6 domain a TRP domain comprising 25 amino acids that is more or less conserved among most TRP channels. Within the TRP domain, there is a TRP box composed of six amino acids, and TRP box 2 – a proline rich domain1,3. The TRP domain seems to be responsible for the binding of PIP2, a phospholipid important for the regulation of channel activity4. TRPP1 (polycystin-2, PC2, PKD2) belongs to the TRPP subfamily of TRP channels along with TRPP3 and TRPP5 proteins, and forms non-selective cation channels with different permeability to various divalent cations5,6. The cellular localization of TRPP1 has been and still is the subject of a lasting debate. In many cell-types, TRPP1 is retained in the endoplasmic reticulum (ER) where it most likely functions as Ca2+ release channel7,8, and with the help of cofactors, TRPP2 reaches the plasma membrane and the cilia8. TRPP1 expression is widespread and is best characterized for its expression in the kidney where it is developmentally regulated9,10. In the kidney, it associates with PKD1 (TRPP2) an eleven transmembrane-spanning protein (which does not belong to the TRP superfamily) to form functional channels11. In addition, TRPP1 is identified as one of the genes responsible for autosomal dominant polycystic kidney disease (ADPKD)5,12. |
| 运输条件 | Ambient |
| 存放说明 | -20 |
| 纯度 | Affinity purified on immobilized antigen. |
| 参考文献 | 1.Ramsey, I.S.et al.(2006)Annu. Rev. Physiol.68,619. 2.Pedersen, S.F.et al.(2005)Cell Calcium38,233. 3.Montell, C.(2005)Sci. STKE2005,re3. 4.Hilgemann, D.W.et al.(2001)Sci. STKE2001,re19. 5.Tsiokas, L.(2009)Am. J. Renal. Physiol.297,F1. 6.Owsianik, G.et al.(2006)Annu. Rev. Physiol.68,685. 7.Koulen, P.et al.(2002)Nat. Cell Biol.4,191. 8.Giamarchi, A.et al.(2006)EMBO Rep.7,787. 9.Wilson, P.D.(2001)J. Am. Soc. Nephrol.12,834. 10.Venkatachalam, K. and Montell, C.(2007)Annu. Rev. Biochem.76,387. 11.Hanaoka, K.et al.(2000)Nature408,990. 12.Mochizuki, T.et al.(1996)Science272,1339. |
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