| 货号 | ABC-001-50ul |
| 描述 | Each antibody ordered from Alomone Labs is supplied with its corresponding control peptide (antigen), free of charge. A Rabbit Polyclonal Antibody to Bestrophin-1 Channel |
| 反应种属 | H, M, R |
| 应用 | IFC, IH, WB |
| 供应商 | Alomone |
| 背景 | MammalianCl- channels can be broadly classified into four different families: voltage-dependent Cl- channels (CLCs), the cystic fibrosis transmembrane conductance regulator (CFTR), ligand-gated Cl- channels (γ-aminobutyric acid (GABA)) and glycine channels) and Ca2+-activated Cl- channels (Bestrophin and Anoctamin channels). Bestrophins were first found by genetic linkage of human-Bestrophin-1 (hBest1) to a juvenile form of macular degeneration called Best vitelliform macular dystrophy (BVMD)1,2. BVMD is mainly electrophysiologically characterized by a decrease in the light peak and physiologically by the thinning of the retina layer which eventually leads to the loss of central vision3. To date Bestrophin 1-4 have been identified, although Bestrophin-3 and Bestrophin-4 have been observed only at the RNA level3. In addition, splice variants of some of these Ca2+-activated Cl- channels (CaCCs) have also been detected2,4,5. CaCCs are known to be involved in the regulation of olfaction, taste, phototransduction, and excitability in the nervous system. Recently, Bestrophin-1 was shown to be functionally expressed in astrocytes in both primary cell culture and in situ6. Bestrophin-1 is also detected in retina, brain, spinal cord and testes7. Two different topologies for Bestrophin-1 have been proposed. The first, the preferred structure, proposes that six hydrophobic domains span the membrane8, while the second suggests that there are only four membrane-spanning domains9. Bestrophin-1, along with its counterparts, is activated by intracellular Ca2+. A recent study demonstrated that Bestrophin-1 indeed binds Ca2+ and by mutating specific residues, showed which amino acid residues are essential for binding Ca2+ 10, providing additional evidence that Bestrophin-1 is activated by direct binding of Ca2+ to the channel11,12. Bestrophin-1 has been found to release glutamate from astrocytes and is located at microdomains near synapses13. |
| 运输条件 | Ambient |
| 存放说明 | -20 |
| 纯度 | Affinity purified on immobilized antigen. |
| 参考文献 | 1.Men, G.et al.(2004)Am. J. Ophtalmol.137,963. 2.Petrukhin, K.et al.(1998)Nat. Genet.19,241. 3.Hartzell, C.H.et al.(2008)Physiol. Rev. 88,639. 4.Kramer, F.et al.(2004)Cytogen. Genome Res.105,107. 5.Stohr, Het al.(2002)Eur. J. Hum. Genet.10,281. 6.Park, H.et al. (2009)J. Neurosci.29,13063. 7.Marmostein, A.D.et al.(2009)Prog Retin Eye Res.28,206. 8.Tsunenari, T.et al.(2003)J. Biol. Chem.278,41114. 9.Milenkovic, V.M.et al.(2007)J. Biol. Chem.282,1313. 10.Xiao, Q.et al.(2008)J. Gen. Physiol.132,681. 11.Chien, L.T.et al.(2006)J. Gen. Physiol.128,247. 12.Tsunenari, T.et al.(2006)J. Gen. Physiol.127,749. 13.Woo, D.H.et al.(2012)Cell151,25. |
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