| 货号 | AQP-002-25ul |
| 描述 | Each antibody ordered from Alomone Labs is supplied with its corresponding control peptide (antigen), free of charge. A Rabbit Polyclonal Antibody to Aquaporin 2 Channel |
| 反应种属 | H, M, R |
| 应用 | IH, WB |
| 供应商 | Alomone |
| 背景 | Aquaporin 2 (AQP-2) belongs to a family of membrane proteins that allow passage of water and certain other solutes through biological membranes. The family is composed of 13 members (AQP-0 to AQP-12). Little is known about the function of the two newest members, AQP-11 and AQP-12. The aquaporins can be divided into two functional groups based on their permability characteristics: the aquaporins that are only permeated by water and the aquaglyceroporins that are permeated by water and other small solutes such as glycerol. AQP-2 together with AQP-1,AQP-4andAQP-5 belongs to the first group.1 The proteins present a conserved structure of six transmembrane domains with intracellular N- and C-termini. The functional channel is a tetramer but each subunit has a separate pore and therefore the functional channel unit, contains four pores.1 AQP-2 expression is largely confined to the kidney, particularly in the renal collecting duct where it performs a key role in water absorption and urine concentration. In fact, mutations in the AQP-2 gene produce hereditary nephrogenic diabetes insipidus, a disorder that results in the excretion of large volumes of urine.2 Under normal conditions, water homeostasis in the kidney is regulated through the anti-diuretic hormone vasopressin. Vasopressin is secreted from the pituitary gland and transported to the kidney through the blood where it binds to its receptor that is mainly expressed in cells of the collecting duct. The activated vasopressing receptor induces an increase in intracellular cAMP and subsequent PKA activation, which phosphorylates AQP-2. This phosphorylation causes the translocation of AQP-2 channels from intracellular vesicles to the cell membrane where it markedly increases water permeability.1, 3, 4 |
| 运输条件 | Ambient |
| 存放说明 | -20 |
| 纯度 | Affinity purified on immobilized antigen. |
| 参考文献 | 1.King, L.S.et al.(2004)Nat. Rev. Mol. Cell Biol.5,687. 2.Deen, P.M.et al. (1994)Science264,92. 3.Nielsen, S.et al.(2002)Physiol. Rev. 82,205. 4.Robben, J.H.et al.(2006)Am. J. Physiol. Renal Physiol. 291,F257. |
| 本官网所有报价均为常温或者蓝冰运输价格，如有产品需要干冰运输，需另外加收干冰运输费。 |