| 货号 | APC-005-25ul |
| 描述 | Each antibody ordered from Alomone Labs is supplied with its corresponding control peptide (antigen), free of charge. A Rabbit Polyclonal Antibody to Kir3.1 (GIRK1) Channel |
| 反应种属 | H, M, R |
| 应用 | IC, IH, IP, WB |
| 供应商 | Alomone |
| 背景 | Kir3.1 (or G-protein regulated Inward-Rectifier K+ channel, GIRK1) is a member of the family of inward rectifying K+ channels. The family includes 15 members that are structurally and functionally different from the voltage-dependent K+channels. The family’s topology consists of two transmembrane domains that flank a single and highly conserved pore region with intracellular N- and C-termini. As is the case for the voltage-dependent K+ channels the functional unit for the Kir channels is composed of four subunit that can assembly as either homo or heterotetramers. Kir channels are characterized by a K+ efflux that is limited by depolarizing membrane potentials thus making them essential for controlling resting membrane potential and K+homeostasis. Kir3.1 is a member of the Kir3.x subfamily that includes four members (Kir3.1- Kir3.4). The Kir3 family is characterized by the fact that the channels can be activated by neurotransmitters and other factors acting via the activation of G-protein coupled receptors. Binding of the corresponding ligand to the G-protein receptor induces the dissociation of Gα-GTP from the Gβγ dimer. The latter directly binds to Kir3 and activates the channel.1,3 In the heart, Kir3.1 co-assembles with Kir3.4 to form the prototypical muscarinic-gated K+ channel KAch current, responsible for slowing the heart rate in response of parasympathetic stimulation.2 In the brain, Kir3.1 co-assembles with Kir3.2 and mediates the inhibitory effects of many neurotransmitters including opioid,adrenergic,muscarinic,dopaminergicandγ-aminobutyric acid(GABA).1,3 A peptide toxin originating from the Apis mellifera bee venom, Tertiapin (#STT-250) was shown to be a potent blocker of Kir3.1 containing channels (8.6 nM for the Kir3.1/3.4 combination and 5.4 nM for the Kir3.1/3.2).4,5 |
| 运输条件 | Ambient |
| 存放说明 | -20 |
| 纯度 | The serum was depleted of anti-GST antibodies by affinity chromatography on immobilized GST, and then the antibody was affinity purified on immobilized GIRK1-GST. |
| 参考文献 | 1.Dascal, N.(1997)Cell Signal9,551. 2.Wickman, K.et al.(1998)Neuron20,103. 3.Mark, M.D.et al.(2000)Eur. J. Biochem.267,5830. 4.Jin, W. and Lu, Z.(1998)Biochemistry37,13291. 5.Kubo, Y.et al.(2005)Pharmacol. Rev.57,509. |
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