| 货号 | AHC-001-25ul |
| 描述 | Each antibody ordered from Alomone Labs is supplied with its corresponding control peptide (antigen), free of charge. A Rabbit Polyclonal Antibody to HVCN1 Channel |
| 反应种属 | H, M, R |
| 应用 | IFC, WB |
| 供应商 | Alomone |
| 背景 | Currents measured from voltage-gated proton channels were detected1 long before the channel (HVCN1, also known as Hv1 and VSOP) was cloned2,3. HVCN1 has four membrane spanning domains and intracellular N- and C-termini. Interesting aspects of HVCN1 is that unlike its voltage-gated ion channel counterparts, it has no pore domain4. Also, functional HVCN1 channels are formed by dimers where each monomer has its own conducting pore, each with its own voltage sensor (voltage sensing occurs similarly to other voltage-gated ion channels)5-8. The fundamental role of HVCN1 is to pump out protons, thereby increasing the intracellular pH. The channel is exclusively selective for H+ and opens upon membrane depolarization, although its open state hugely depends on the pH on both sides of the membrane4. Its role is best described in leukocytes where phosphorylation via PKC on a Thr residue potentiates the activity of the channel and increases its open state, thereby increasing the H+ current across the membrane, in this manner mediating optimal NADPH-oxidase (whose optimal activity is at pH 7.5) required for the production of reactive oxygen species (ROS) necessary for phagocytosis to occur4. Apart from leukocytes, HVCN1 is also expressed in basophils where its activation mediates histamine release4,9. In B cells, it maintains optimal signaling, such that ROS production is maintained high10. HVCN1 was found to regulate human spermatozoa activation. Finally, in the airway mucosa, where it regulates pH, channel gating there, is mostly mediated by differences in pH across the membrane as opposed to the membrane potential4. |
| 运输条件 | Ambient |
| 存放说明 | -20 |
| 纯度 | Affinity purified on immobilized antigen. |
| 参考文献 | 1.DeCoursey, T.E.(1991)Biophys. J.60,1243. 2.Ramsey, I.S.et al.(2006)Nature440,1213. 3.Sasaki, M.et al.(2006)Science312,589. 4.Capasso, M.et al.(2011)Trends Cell Biol.21,20. 5.Koch, H.P.et al.(2008)Proc. Natl. Acad. Sci. U.S.A.105,9111. 6.Lee, S.Y.et al.(2008)Proc. Natl. Acad. Sci. U.S.A.105,7692. 7.Tombola, F.et al.(2008)Neuron58,546. 8.Demaurex, N. and El Chemaly, A.(2010)J. Physiol.588.23,4659. 9.Musset, B.et al.(2008)Proc. Natl. Acad. Sci. U.S.A.105,11020. 10.Schilling, T.et al.(2002)J. Physiol.545,93. |
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