| 货号 | AGC-021-25ul |
| 描述 | Each antibody ordered from Alomone Labs is supplied with its corresponding control peptide (antigen), free of charge. A Rabbit Polyclonal Antibody to Excitatory Amino Acid Transporter 1 |
| 反应种属 | M, R |
| 应用 | IC, IH, LCI, WB |
| 供应商 | Alomone |
| 背景 | L-Glutamate (Glu) is an abundant amino acid that functions as the major excitatory neurotransmitter in the central nervous system. However, excess of Glu in the extracellular synaptic milieu leads to neuronal cell death by a process known as excitotoxicity. The extracellular levels of Glu are regulated by a family of high affinity plasma membrane transporters called excitatory amino acid transporters (EAATs) which are responsible for re-uptake of Glu into the cells.1,2 TheEAAT family includes five members (EAAT1-EAAT5) that are members of the solute carrier family 1 (SLC1) of sodium-dependent transporters that also includes the neutral amino acid transporters ASCT1 and ASCT2. The Glu transporters present an unusual topology of eight transmembrane domains with two re-entrant loops and intracellular N- and C- termini. The transporter is likely assembled as a trimer where each monomer is a functional unit capable of binding the Glu substrate. The transport of Glu into the cells by the EAAT transporters is coupled to the Na+ and K+ electrochemical gradient as a driving force. Hence, the uptake of Glu is dependent on the co-transport of three Na+ and one H+ ions, and the counter transport of one K+ ion. In addition, to the well documented Glu uptake, the EAAT transporters show a Glu-independent Cl- conductance. The physiological significance of the Cl- current through the EAATs is currently unknown.1,2 EAAT1 as well as EAAT2, is expressed predominantly in glia cells (hence its original name: glial glutamate transporter or GLAST), while EAAT3,EAAT4 and EAAT5 are mostly expressed in neurons. As mentioned earlier, EAAT transporters represent the only (significant) mechanism for removal of glutamate from the extracellular fluid and hence are essential for the long-term maintenance of low and non-toxic concentrations of glutamate and the preservation of normal excitatory synaptic transmission. In addition, to Glu uptake the glutamate transporters provide glutamate for the synthesis of γ-Aminobutyric acid (GABA), glutathione and protein, suggesting an interactive role between EAATs and cellular metabolism.1,2 Dysregulation of EAATs activities has been implicated in several neurodegenerative disorders such as Alzheimer’s disease, traumatic brain injury, epilepsy and schizophrenia, suggesting that EAATs can be a useful target for the treatment of these conditions.1,2 |
| 运输条件 | Ambient |
| 存放说明 | -20 |
| 纯度 | Affinity purified on immobilized antigen. |
| 参考文献 | 1.Tzingounis, A.V. and Wadiche, J.I.(2007)Nat. Rev. Neurosci. 8,935. 2.Beart, P.M. and O’Shea, R.D.(2007)Br. J. Pharmacol.150,5. |
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