| 货号 | AF7079 |
| 别名 | BoNTG Light Chain; BoNT-G Light Chain | 全称 | Botulinum Neurotoxin Type G Light Chain |
| 应用 | Western Blot(1 µg/mL) |
| 目标/特异性 | DetectsC. botulinum BoNT-G Light Chain in direct ELISAs and Western blots. In direct ELISAs, less than 2% cross-reactivity with recombinant BoNT-A/LC, -B/LC, -C/LC, -D/LC, -E/LC, and BoNT-F/LC is observed. |
| 使用方法 | Western Blot: 1 µg/mL |
| 来源 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 产品组分 | |
| 是否允许进口 | 否 |
| 是否危险品 | 是 |
| 供应商 | R&D Systems |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant C. botulinum BoNT-G Light Chain Pro2-His427 Accession # Q60393 |
| 生物活性 | C. botulinum |
| 标记 | Unconjugated |
| 溶解方法 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 背景 | BoNT-G LC (Botulinum neurotoxin serotype G light chain) is a 47 kDa member of the peptidase M27 family of molecules. It is the product of Clostridium botulinum, and inhibits neurotransmitter release from the neuromuscular junction. This is accomplished by BoNT-G binding to synaptotagmin and ganglioside, followed by its internalization and subsequent light chain-mediated cleavage of synaptobrevin/VAMP that blocks synaptic vesicle fusion with the presynaptic membrane. BoNT-G precursor is 1297 amino acids (aa) in length. Following internalization and precursor proteolytic cleavage, it assumes a mature form that contains a 442 aa N-terminal enzymatic light chain disulfide-linked to an 855 aa C-terminal receptor-binding heavy chain. The 97 kDa heavy chain creates a channel within the endosome that allows for redox rupture of the disulfide bond and entry of the enzymatic light chain into the cytosol where it generates noncovalent homodimers. Over aa 2-427, BoNT-G light chain shares 60% aa identity with BoNT-B light chain. |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
| 参考文献 |
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Detection of C. botulinum BoNT‑G Light Chain by Western Blot. Western blot shows recombinant C. botulinum BoNT-G Light Chain. PVDF membrane was probed with 1 µg/mL of Sheep Anti-C. botulinum BoNT‑G Light Chain Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7079) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for BoNT‑G Light Chain at approximately 52 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
