C. difficile Toxin B/TcdB Affinity Purified Polyclonal Ab (100 UG)【科瑞奥博】

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C. difficile Toxin B/TcdB Affinity Purified Polyclonal Ab (100 UG)

货号： AF6246 基本售价：询价规格： -

产品信息

概述
货号	AF6246
别名	toxB
应用	Western Blot(1 µg/mL)
目标/特异性	DetectsC. difficile Toxin B/TcdB in direct ELISAs and Western blots.
使用方法	Western Blot: 1 µg/mL
来源	Sterile PBS to a final concentration of 0.2 mg/mL.
产品组分
是否允许进口	否
是否危险品	是

性能
供应商	R&D Systems
Entrez Gene IDs	4914074 (C. difficile)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Amino Acid Differences in the 1753-to-1851 Region of TcdB Influence Variations in TcdB1 and TcdB2 Cell Entry Authors: JJ Hunt, JL Larabee, JD Ballard mSphere, 2017;2(4):. Species: Chinese Hamster Sample Type: Cell Lysates Application: WB Intrinsic Toxin-Derived Peptides Destabilize and Inactivate Clostridium difficile TcdB Authors: JL Larabee, SJ Bland, JJ Hunt, JD Ballard MBio, 2017;8(3):. Species: Hamster Sample Type: Cell Lysates Application: WB
纯化方式	Antigen Affinity-purified
免疫原	E. coli-derived recombinant C. difficile Toxin B/TcdB Ser2-Leu543 Accession # P18177
生物活性	C. difficile
标记	Unconjugated
溶解方法	Sterile PBS to a final concentration of 0.2 mg/mL.
背景	Clostridium difficile is the leading cause of hospital-acquired diarrhea, known as C. difficile-associated disease. The estimated number of cases of C. difficile-associated disease exceeds 250,000 per year (1), with health care costs approaching US $1 billion annually (2). The major virulence factors produced by C. difficile are two toxins, TcdA and TcdB. Both toxins can monoglucosylate and inactivate Rho family small GTPases within target cells, leading to disruption of vital signaling pathways in the cell, subsequently causing diarrhea, inflammation, and damage of colonic mucosa (3, 4, 5). Both toxins have a similar tripartite structure comprised of an N‑terminal glucosyltransferase domain, a C-terminal receptor binding domain, and a small hydrophobic span possibly involved in toxin translocation (6). Our recombinant TcdB consists of the enzymatic domain. Both TcdA and TcdB also have potassium-dependent UDP-Glc hydrolase activity, which is essentially glucosyltransferase activity with water as the acceptor molecule (7). Under same conditions, UDP-glucose hydrolysis by TcdB occurs at a rate about 5-fold greater than that of TcdA.
运输条件	Blue Ice
存放说明	-20℃
参考文献	Wilkins, T.D. and Lyerly, D.M. (2003) J. Clin. Microbiol 41:53. Kyne, L. et al. (2002) Clin. Infect. Dis. 34:346. Voth, D.E. and Ballard, J.D. (2005) Clin. Microbiol. Rev. 18:247. Chaves-Olarte, E. et al. (1996) J. Biol. Chem. 271:6925. Just I, et al. (1995) J. Biol. Chem. 270:13932. Hammond, G.A. and Johnson, J.L. (1995) Microb. Pathog. 19:203. Ciesla, W.P. Jr. and Bobak, D.A. (1998) J. Biol. Chem. 273:16021.

参考图片

Detection of C. difficile Toxin B/TcdB by Western Blot. Western blot shows recombinant C. difficile Toxin B/TcdB (Catalog # 6246-GT). PVDF membrane was probed with 1 µg/mL of Sheep Anti-C. difficile Toxin B/TcdB Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6246) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Toxin B/TcdB at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.