| 货号 | AF7135 |
| 别名 | Bontoxilysin-E | 全称 | Botulinum Neurotoxin Type E Heavy Chain |
| 应用 | Western Blot(1 µg/mL) |
| 目标/特异性 | DetectsC. botulinum BoNT-E Heavy Chain in direct ELISAs and Western blots. In direct ELISAs, approximately 4% cross-reactivity with BoNT-G/HCR and BoNT-A/HCR is observed. |
| 使用方法 | Western Blot: 1 µg/mL |
| 来源 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 产品组分 | |
| 是否允许进口 | 否 |
| 是否危险品 | 是 |
| 供应商 | R&D Systems |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant C. botulinum BoNT-E Heavy Chain Lys845-Lys1251 Accession # P30995 |
| 生物活性 | C. botulinum |
| 标记 | Unconjugated |
| 溶解方法 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 背景 | BoNT/E HCR (Botulinum neurotoxin serotype E heavy chain receptor) is a 105 kDa component of BoNT/E, a member of the peptidase M27 family of molecules. BoNT/E is the product of Clostridium botulinum, and inhibits neurotransmitter release from neuromuscular junctions. This is accomplished by toxin internalization with subsequent cleavage of membrane-associated SNAP25, thus blocking synaptic vesicle fusion with the presynaptic membrane. The 155 kDa BoNT/E precursor is 1251 amino acids (aa) in length. Following internalization and precursor proteolytic cleavage by host proteases, it assumes a mature form that contains a 50 kDa disulfide-linked 422 aa light chain/enzyme N-terminus, and a 105 kDa, 829 aa heavy chain/receptor-binding C-terminus. The heavy chain has two components, a 327 aa translocation domain (aa 518-840), and a 408 aa cell membrane SV2:ganglioside binding region (aa 844-1250). The heavy chain creates a channel within the endosome that allows for redox rupture of the disulfide bond and entry of the light chain into the cytosol. The E heavy chain shares less that 50% aa identity with the BoNT/A heavy chain. |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
| 参考文献 |
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Detection of C. botulinum BoNT-E Heavy Chain by Western Blot. Western blot shows recombinant C. botulinum BoNT-E Heavy Chain (10 ng/lane). PVDF membrane was probed with 1 µg/mL of Sheep Anti-C. botulinum BoNT-E Heavy Chain Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7135) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for BoNT-E Heavy Chain at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8. |
