| 货号 | AF5425 |
| 别名 | BoNTC1 Heavy Chain; BoNT-C1 Heavy Chain | 全称 | Botulinum Neurotoxin Type C1 Heavy Chain |
| 应用 | Western Blot(0.1 µg/mL) Immunoprecipitation(25 µg/mL) |
| 目标/特异性 | DetectsC. botulinum BoNT-C1 Heavy Chainin direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 10% cross‑reactivity with recombinant (r) BoNT‑D‑HCR is observed and 5% cross-reactivity with rBoNT-A-HCR, rBoNT-A-LC, and rBoNT‑B‑LC is observed. |
| 使用方法 | Western Blot: 0.1 µg/mL Immunoprecipitation: 25 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 | |
| 是否允许进口 | 否 |
| 是否危险品 | 是 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 10523893 (C. botulinum) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant BoNT-C1 Heavy Chain Asn866-Glu1291 Accession # P18640 |
| 生物活性 | C. botulinum |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | BoNT-C1 (Botulinum neurotoxin C1 [heavy chain]) is a 100 kDa C-terminal fragment of the C1 Clostridum botulinum neurotoxin (holo)precursor. It constitutes a nonenzymatic part of a Zn2+-dependent M27 family endoprotease that potently blocks neurosecretion. The inactive 1291 amino acid (aa), 150 kDa C1 precursor is cleaved between Lys449Thr450 to generate a heterodimeric toxin held together by a preexisting interchain disulfide bond between Cys437 and Cys453. The 100 kDa C-terminus (heavy chain) acts as a ligand for neuronal membrane ecto-acceptors, inducing its translocation and internalization. The 50 kDa N-terminal light chain acts as an intracellular peptidase that cleaves SNAP-25 and syntaxin. This is suggested to block noradrenalin release from a readily releasable pool of vesicles. Over aa 866-1291, the C1 heavy chain shares less than 40% aa identity with the D-type neurotoxin heavy chain. |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
| 参考文献 |
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