| 货号 | AB-101-NA |
| 别名 | TGFb1.2; TGF-beta 1/1.2 | 全称 | Transforming Growth Factor beta 1 and 1.2 |
| 应用 | Western Blot(1 µg/mL) |
| 目标/特异性 | Detects TGF‑ beta 1/1.2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 10% cross-reactivity with recombinant amphibian TGF‑ beta 5 is observed and less than 2% cross-reactivity with recombinant human TGF‑ beta 3, recombinant chicken TGF‑ beta 3, and porcine TGF‑ beta 2 is observed. |
| 使用方法 | Western Blot: 1 µg/mL Neutralization: Measured by its ability to neutralize TGF‑ beta 1 inhibition of IL‑4-dependent proliferation in the HT‑2 mouse T cell line [Tsang, M. et al. (1995) Cytokine 7:389]. The Neutralization Dose (ND50) is typically 0.2-0.6 µg/mL in the presence of 0.25 ng/mL Recombinant Human TGF‑ beta 1 and 7.5 ng/mL Recombinant Mouse IL‑4. |
| 来源 | Polyclonal Chicken IgY |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 7040 (Human); 21803 (Mouse); 59086 (Rat) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Isoform specificity of commercially-available anti-TGF-beta antibodies. | |
| 纯化方式 | Ion exchange-purified from egg yolks |
| 免疫原 | Chinese hamster ovary cell line CHO-derived recombinant human TGF‑ beta 1 (R&D Systems, Catalog # 240-B) Ala279-Ser390 Accession # P01137 |
| 内毒素水平 | <0.10 EU per 1 μg of the antibody by the LAL method. |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 1 mg/mL in sterile PBS. |
| 背景 | TGF-beta 1 (transforming growth factor beta 1) is one of three closely related mammalian members of the large TGF-beta superfamily that share a characteristic cystine knot structure. TGF-beta 1, -2 and -3 are highly pleiotropic cytokines that are proposed to act as cellular switches that regulate processes such as immune function, proliferation and epithelial-mesenchymal transition. Each TGF-beta isoform has some non-redundant functions; for TGF-beta 1, mice with targeted deletion show defects in hematopoiesis and endothelial differentiation, and die of overwhelming inflammation. Human TGF-beta 1 cDNA encodes a 390 amino acid (aa) precursor that contains a 29 aa signal peptide and a 361 aa proprotein. A furin-like convertase processes the proprotein to generate an N-terminal 249 aa latency-associated peptide (LAP) and a C-terminal 112 aa mature TGF- beta 1. Disulfide-linked homodimers of LAP and TGF-beta 1 remain non-covalently associated after secretion, forming the small latent TGF-beta 1 complex. Covalent linkage of LAP to one of three latent TGF-beta binding proteins (LTBPs) creates a large latent complex that may interact with the extracellular matrix. TGF-beta is activated from latency by pathways that include actions of the protease plasmin, matrix metalloproteases, thrombospondin 1 and a subset of integrins. Mature human TGF-beta 1 shares 100% aa identity with pig, dog and cow TGF-beta 1, and 99% aa identity with mouse, rat and horse TGF-beta 1. It demonstrates cross-species activity. TGF-beta 1 signaling begins with high-affinity binding to a type II ser/thr kinase receptor termed TGF-beta RII. This receptor then phosphorylates and activates a second ser/thr kinase receptor, TGF-beta RI (also called activin receptor-like kinase (ALK) -5), or alternatively, ALK‑1. This complex phosphorylates and activates Smad proteins that regulate transcription. Contributions of the accessory receptors betaglycan (also known as TGF-beta RIII) and endoglin, or use of Smad-independent signaling pathways, allow for disparate actions observed in response to TGF-beta in different contexts. |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
| 参考文献 |
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TGF‑ beta 1 Inhibition of |
