Mouse CCL4/MIP-1 beta Biotinylated Affinity Purified PAb (50 UG)【科瑞奥博】

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Mouse CCL4/MIP-1 beta Biotinylated Affinity Purified PAb (50 UG)

货号： BAF451 基本售价： 6077.6 元规格： -

产品信息

概述
货号	BAF451
别名	ACT2; ACT-2; AT744.1; C-C motif chemokine 4; chemokine (C-C motif) ligand 4; Exodus-3; G-26 T-lymphocyte-secreted protein; G-26; HC21; LAG1; LAG-1; Lymphocyte activation gene 1 protein; Macrophage inflammatory protein 1-beta; MIP-1 beta; MIP1B; MIP1B1; MIP-1-beta; MIP-1-beta(1-69); PAT 744; Protein H400; SCYA2; SCYA4; secreted protein G-26; SIS-gamma; small inducible cytokine A4 (homologous to mouse Mip-1b); Small-inducible cytokine A4; T-cell activation protein 2
反应种属	Mouse
应用	Western Blot(0.1 µg/mL) Immunohistochemistry(5-15 µg/mL)
目标/特异性	Detects mouse CCL4/MIP-1 beta in Western blots. In this format, less than 1% cross-reactivity with recombinant human (rh) RANTES, rhMIP-1 beta, recombinant mouse (rm) MIP-1 alpha, rhIL-8, rhGRO alpha, rhGRO beta, rhGRO gamma, rhENA‑78, rmMIP‑2, rhMCP‑1, rhMCP-2, rhMCP‑3, rhI-309, rmJE, rmC10, and rhIP-10 is observed.
使用方法	Western Blot: 0.1 µg/mL Immunohistochemistry: 5-15 µg/mL
来源	Reconstitute at 0.2 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	6351 (Human); 20303 (Mouse); 116637 (Rat); 448786 (Canine)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Regulatory effects of eotaxin on acute lung inflammatory injury. Authors: Guo RF, Lentsch AB, Warner RL, Huber-Lang M, Sarma JV, Hlaing T, Shi MM, Lukacs NW, Ward PA J. Immunol., 2001;166(8):5208-18. Species: Mouse Sample Type: Cell Culture Supernates Application: ELISA Development Role of CC chemokines (macrophage inflammatory protein-1 beta, monocyte chemoattractant protein-1, RANTES) in acute lung injury in rats. Authors: Bless NM, Huber-Lang M, Guo RF, Warner RL, Schmal H, Czermak BJ, Shanley TP, Crouch LD, Lentsch AB, Sarma V, Mulligan MS, Friedl HP, Ward PA J. Immunol., 2000;164(5):2650-9. Species: Rat Sample Type: BALF Application: ELISA Development
纯化方式	Antigen Affinity-purified
免疫原	E. coli-derived recombinant mouse CCL4/MIP-1 beta (R&D Systems, Catalog # 451-MB) Ala24-Asn92 Accession # Q5QNV9
生物活性	Mouse
标记	Biotin
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	CCL4, also known as macrophage inflammatory protein 1 beta (MIP-1 beta ), is a 12 kDa beta chemokine that is secreted at sites of inflammation by activated leukocytes, lymphocytes, vascular endothelial cells, and pulmonary smooth muscle cells (1, 2). CCL4 attracts a variety of immune cells to sites of microbial infection as well as to other pathologic inflammation such as allergic asthma and ischemic myocardium (3-8). A CCL4 deficiency in mice promotes the development of autoantibodies, possibly as a result of compromised regulatory T cell recruitment (6). CCL4 is secreted from activated monocytes as a heterodimer with CCL3/MIP-1 alpha (9). The first two N-terminal amino acids can be cleaved from human CCL4 by CD26/DPPIV (10, 11). Both the full length and truncated forms exert biological activity through CCR5, and the truncated form additionally interacts with CCR1 and CCR2 (10). In humans, the ability of CCL4 to bind CCR5 inhibits the cellular entry of M-tropic HIV-1 which utilizes CCR5 as a coreceptor (2). Both forms of CCL4 block HIV-1 infection of T cells by inducing the downregulation of CCR5 (10). Mature mouse CCL4 shares 77% and 86% aa sequence identity with human and rat CCL4, respectively.
运输条件	Blue Ice
存放说明	-20℃
参考文献	Rot, A. and U.H. von Andrian (2004) Annu. Rev. Immunol. 22:891. Menten, P. et al. (2002) Cytokine Growth Factor Rev. 13:455. Sun, X. et al. (2006) Infec. Immun. 74:5943. Bisset, L.R. and Schmid-Grendelmeier, P. (2005) Curr. Opin. Pulm. Med. 11:35. Frangogiannis, N.G. (2004) Inflamm. Res. 53:585. Bystry, R.S. et al. (2001) Nat. Immunol. 2:1126. Oliveira, S.H.P. et al. (2002) J. Leukoc. Biol. 71:1019. Schall, T.J. et al. (1993) J. Exp. Med. 177:1821. Guan, E. et al. (2001) J. Biol. Chem. 276:12404. Guan, E. et al. (2002) J. Biol. Chem. 277:32348. Guan, E. et al. (2004) J. Cell. Biochem. 92:53.

参考图片

CCL4/MIP‑1 beta in Mouse Spleen. CCL4/MIP‑1 beta was detected in immersion fixed frozen sections of mouse spleen using Mouse CCL4/MIP‑1 beta Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF451) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.