Mouse CCL11/Eotaxin Biotinylated Affinity Purified PAb (50 UG)【科瑞奥博】

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Mouse CCL11/Eotaxin Biotinylated Affinity Purified PAb (50 UG)

货号： BAF420 基本售价： 6077.6 元规格： -

产品信息

概述
货号	BAF420
描述	For ELISA the Antibody Pairs information：Capture antibody：AF-420-NA；Detection antibody：BAF420; and protein: 420-ME-020
别名	C-C motif chemokine 11; chemokine (C-C motif) ligand 11; Eosinophil chemotactic protein; Eotaxin; eotaxin-1; MGC22554; SCYA11Small-inducible cytokine A11; small inducible cytokine subfamily A (Cys-Cys), member 11 (eotaxin)
反应种属	Mouse
应用	Western Blot(0.1 µg/mL) Immunohistochemistry(5-15 µg/mL) ELISA Capture (Matched Antibody Pair)(0.2-0.8 µg/mL ) ELISA Detection (Matched Antibody Pair)(0.1-0.4 µg/mL ) ELISA Standard ( )
目标/特异性	Detects mouse CCL11/Eotaxin in ELISAs and Western blots. In sandwich immunoassays, less than 0.5% cross-reactivity with recombinant human CCL11/Eotaxin and recombinant mouse CCL24/Eotaxin-2 is observed.
使用方法	Western Blot: 0.1 µg/mL Immunohistochemistry: 5-15 µg/mL ELISA Capture (Matched Antibody Pair): 0.2-0.8 µg/mL ELISA Detection (Matched Antibody Pair): 0.1-0.4 µg/mL ELISA Standard :
来源	Reconstitute at 0.2 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	6356 (Human); 20292 (Mouse)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Response patterns of cytokines/chemokines in two murine strains after irradiation. Authors: Zhang M, Yin L, Zhang K, Sun W, Yang S, Zhang B, Salzman P, Wang W, Liu C, Vidyasagar S, Zhang L, Ju S, Okunieff P, Zhang L Cytokine, 2012;58(2):169-77. Species: Mouse Sample Type: Plasma Application: Luminex Assay Development Resolution of allergic inflammation and airway hyperreactivity is dependent upon disruption of the T1/ST2-IL-33 pathway. Authors: Kearley J, Buckland KF, Mathie SA, Lloyd CM Am. J. Respir. Crit. Care Med., 2009;179(9):772-81. Species: Mouse Sample Type: Cell Culture Supernates Application: ELISA Development IL-13Ralpha2 and IL-10 coordinately suppress airway inflammation, airway-hyperreactivity, and fibrosis in mice. Authors: Wilson MS, Elnekave E, Mentink-Kane MM, Hodges MG, Pesce JT, Ramalingam TR, Thompson RW, Kamanaka M, Flavell RA, Keane-Myers A, Cheever AW, Wynn TA J. Clin. Invest., 2007;117(10):2941-51. Species: Mouse Sample Type: Serum Application: ELISA Development Circulating cytokine/inhibitor profiles reshape the understanding of the SIRS/CARS continuum in sepsis and predict mortality. Authors: Osuchowski MF, Welch K, Siddiqui J, Remick DG J. Immunol., 2006;177(3):1967-74. Species: Mouse Sample Type: Plasma Application: ELISA Development Sequential ELISA to profile multiple cytokines from small volumes. Authors: Osuchowski MF, Siddiqui J, Copeland S, Remick DG J. Immunol. Methods, 2005;302(1):172-81. Species: Mouse Sample Type: Plasma Application: ELISA Development P-selectin mediates IL-13-induced eosinophil transmigration but not eotaxin generation in vivo: a comparative study with IL-4-elicited responses. Authors: Larbi KY, Dangerfield JP, Culley FJ, Marshall D, Haskard DO, Jose PJ, Williams TJ, Nourshargh S J. Leukoc. Biol., 2003;73(1):65-73. Species: Mouse Sample Type: Peritoneal Fluid Application: ELISA Development A soluble chemokine-binding protein from vaccinia virus reduces virus virulence and the inflammatory response to infection. Authors: Reading PC, Symons JA, Smith GL J. Immunol., 2003;170(3):1435-42. Species: Mouse Sample Type: BALF Application: ELISA Development AMD3100, a CxCR4 antagonist, attenuates allergic lung inflammation and airway hyperreactivity. Authors: Lukacs NW, Berlin A, Schols D, Skerlj RT, Bridger GJ Am. J. Pathol., 2002;160(4):1353-60. Species: Mouse Sample Type: Tissue Homogenates Application: ELISA Development
纯化方式	Antigen Affinity-purified
免疫原	E. coli-derived recombinant mouse CCL11/Eotaxin (R&D Systems, Catalog # 420-ME) His24-Pro97 Accession # P48298
生物活性	Mouse
标记	Biotin
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	CCL11 is a potent eosinophil chemoattractant that was originally purified from bronchoalveolar lavage fluid of guinea pigs sensitized by aerosol challenge with ovalbumin. Microsequencing of the purified protein revealed the guinea pig CCL11 to be a member of the beta (CC) chemokine family of inflammatory and immunoregulatory cytokines. cDNA clones for guinea pig, mouse and human CCL11 have been isolated. Mouse CCL11 cDNA encodes a 97 amino acid residue precursor protein from which the amino-terminal 23 amino acid residues are cleaved to generate the 74 amino acid residue mature mouse CCL11. At the protein sequence level, mature mouse CCL11 is approximately 60% identical to mature human and guinea pig CCL11. In addition, mouse CCL11 also shows high amino acid sequence identity to members of the MCP family. Mouse CCL11 is chemotactic for eosinophils, but not mononuclear cells or neutrophils. CCL11 mRNA is expressed in a variety of tissues. The expression of CCL11 mRNA is induced in cultured endothelial cells in response to IFN-gamma. In addition, CCL11 mRNA is also induced in response to the transplantation of IL-4-secreting tumor cells. The CC chemokine receptor 3 (CCR3) has now been identified to be a specific human CCL11 receptor.
运输条件	Blue Ice
存放说明	-20℃
参考文献	Rothenberg, M.E. et al. (1995) Proc. Natl. Acad. Sci. USA 92:8960. Kitaura, M. et al. (1996) J. Biol. Chem 271:7725. Garcia-Zepeda, E.A. et al. (1996) Nature Medicine 2:449. Ponath, P.D. et al. (1996) J. Clin. Invest. 97:604.

参考图片

CCL11/Eotaxin in Mouse Colon. CCL11/Eotaxin was detected in perfusion fixed frozen sections of mouse colon using Goat Anti-Mouse CCL11/Eotaxin Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF420) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.