| 货号 | BAF1815 |
| 描述 | For ELISA the Antibody Pairs information：Capture antibody：AF1815；Detection antibody：BAF1815; and protein: 1815-CL-020 |
| 别名 | aldesleukin; IL-2lymphokine; interleukin 2; interleukin-2; involved in regulation of T-cell clonal expansion; T cell growth factor; T-cell growth factor; TCGF | 全称 | Interleukin 2 |
| 反应种属 | Canine |
| 应用 | Western Blot(0.1 µg/mL) Immunocytochemistry(5-15 µg/mL) ELISA Capture (Matched Antibody Pair)(0.2-0.8 µg/mL ) ELISA Detection (Matched Antibody Pair)(0.1-0.4 µg/mL ) ELISA Standard ( ) |
| 目标/特异性 | Detects canine IL-2 in ELISAs and Western blots. In sandwich immunoassays, less than 0.2% cross-reactivity with recombinant human IL-2, recombinant mouse IL-2, recombinant rat IL-2, recombinant feline IL-2, recombinant bovine IL-2, recombinant equine IL-2, recombinant cotton rat IL-2, and recombinant porcine IL-2 is observed. |
| 使用方法 | Western Blot: 0.1 µg/mL Immunocytochemistry: 5-15 µg/mL ELISA Capture (Matched Antibody Pair): 0.2-0.8 µg/mL ELISA Detection (Matched Antibody Pair): 0.1-0.4 µg/mL ELISA Standard : |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 3558 (Human); 16183 (Mouse); 116562 (Rat); 396868 (Porcine); 280822 (Bovine); 403989 (Canine); 100034204 (Equine); 751114 (Feline); 100302458 (Rabbit) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant canine IL‑2 Ala21-Thr155 (Cys147Ser) Accession # Q29416 |
| 生物活性 | Canine |
| 标记 | Biotin |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Interleukin 2 was initially identified as a T cell growth factor that is produced by T cells following activation by mitogens or antigens. Since then, it has been shown that IL-2 can also stimulate the growth and differentiation of B cells, natural killer (NK) cells, lymphocyte activated killer (LAK) cells, monocytes/macrophages, and oligodendrocytes. The biological activity of IL-2 is mediated by the binding to cell surface receptor complexes composed of three subunits designated as alpha, beta, and gamma subunits. IL-2 binds the alpha subunit with low affinity. The functional high affinity IL-2 receptor is a heterotrimeric complex of the alpha, beta, and gamma subunits. IL-2 binds with intermediate affinity to the complex containing the beta and gamma subunits, which is also capable of transducing IL-2 signals. In T cells, the beta and gamma subunits are shared with the IL-15 receptor complex. The gamma subunit of the IL-2 receptor complex has also been shown to be a subunit of the receptor complexes of IL-4, IL-7, and IL-9. At the amino acid sequence level, canine IL-2 shares 90%, 86%, 85%, 76%, and 75% sequence similarities to feline, human, equine, mouse, and bovine IL-2, respectively. |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
| 参考文献 |
|
IL‑2 in Canine PBMCs. IL‑2 was detected in immersion fixed canine peripheral blood mononuclear cells (PBMCs) untreated or treated with calcium ionomycin and PMA using Goat Anti-Canine IL‑2 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF1815) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (red; Catalog # NL999) and counterstained with DAPI (blue). Specific staining was localized to cell secretion. View our protocol for Fluorescent ICC Staining of Non-adherent Cells. |
