| 货号 | BAF442 |
| 描述 | For ELISA the Antibody Pairs information：Capture antibody：MAB442；Detection antibody：BAF442; and protein: 442-LK-002 |
| 别名 | Il8; Lungkine; Scyb15; Weche |
| 反应种属 | Mouse |
| 应用 | Western Blot(0.1 µg/mL) ELISA Capture (Matched Antibody Pair)(2-8 µg/mL ) ELISA Detection (Matched Antibody Pair)(0.1-0.4 µg/mL ) ELISA Standard ( ) |
| 目标/特异性 | Detects mouse CXCL15/Lungkine in ELISAs and Western blots. In sandwich immunoassays, less than 0.5% cross-reactivity with recombinant mouse (rm) BLC/CXCL13, recombinant human (rh) BLC/CXCL13, rhENA-78/CXCL5, rhIL-8/CXCL8, rmMIP-2, recombinant rat CINC-2 beta, rmFractalkine, rmMCP-5 and rhMIP-1δ/CCL15 is observed. |
| 使用方法 | Western Blot: 0.1 µg/mL ELISA Capture (Matched Antibody Pair): 2-8 µg/mL ELISA Detection (Matched Antibody Pair): 0.1-0.4 µg/mL ELISA Standard : |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 20309 (Mouse) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Circulating CXC-chemokine concentrations in a murine intestinal ischemia-reperfusion model. | |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant mouse CXCL15/Lungkine Gln26-Ala167 Accession # Q9WVL7 |
| 生物活性 | Mouse |
| 标记 | Biotin |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Mouse Lungkine/CXCL15 (1), also named WECHE (2), is a member of the ELR motif-containing CXC chemokines. The cDNA of mouse Lungkine encodes a protein of 166 amino acids (aa) with a 25 aa predicted signal peptide and a 141 aa mature protein with an extremely long C terminal tail that protrudes beyond the chemokine fold. Mouse Lungkine shares 35% aa sequence identity with human ENA-78 and 31% identity with human IL-8. The gene for mouse Lungkine has been mapped to chromosome 5. By Northern blot and in situ hybridization, Lungkine transcripts are only specifically detected in the adult and fetal lung, and its expression is up‑regulated under inflammatory conditions (1). Lungkine protein is secreted into bronchoalveolar space and is involved in lung-specific neutrophils trafficking (1). Studies from Lungkine knock out mice suggests that Lungkine is an important mediator of neutrophil migration from the lung parenchyma into the airspace (3). Lungkine is also chemotactic for bone marrow progenitor cells and modulates hematopoietic cell differentiation (2). |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
| 参考文献 |
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