| 货号 | BAF428 |
| 描述 | For ELISA the Antibody Pairs information：Capture antibody：MAB428-500；Detection antibody：BAF428; and protein: 428-P5-025 |
| 别名 | C-C motif chemokine 12; chemokine (C-C motif) ligand 12; MCP-5; Scya12 |
| 反应种属 | Mouse |
| 应用 | Western Blot(0.1 µg/mL) ELISA Capture (Matched Antibody Pair)(2-8 µg/mL ) ELISA Detection (Matched Antibody Pair)(0.1-0.4 µg/mL ) ELISA Standard ( ) |
| 目标/特异性 | Detects mouse CCL12/MCP-5 in ELISAs and Western blots. In sandwich immunoassays, less than 0.07% cross-reactivity with recombinant human (rh) MCP‑1, rhMCP-2, rhMCP-3, rhMCP-4, recombinant mouse (rm) MIP‑1 gamma, rmJE, and rmMARC is observed. |
| 使用方法 | Western Blot: 0.1 µg/mL ELISA Capture (Matched Antibody Pair): 2-8 µg/mL ELISA Detection (Matched Antibody Pair): 0.1-0.4 µg/mL ELISA Standard : |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 20293 (Mouse) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant mouse CCL12/MCP-5 (R&D Systems, Catalog # 428-P5) Gly23-Gly104 Accession # Q62401 |
| 生物活性 | Mouse |
| 标记 | Biotin |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | CCL12 is a cloned mouse CC chemokine most closely related to human MCP-1 (66% amino acid sequence identity in the mature protein). Mouse CCL12 encodes a 104 amino acid residue precursor protein with a 22 aa residue predicted hydrophobic signal sequence that is cleaved to generate a 82 aa residue mature protein. CCL12 is expressed constitutively in the thymus and lymph nodes. Under inflammatory conditions, CCL12 expression is also induced in activated macrophages and mast cells. The gene for mouse MCP-1 has been mapped to the CC chemokine cluster on chromosome 11. Recombinant CCL12 has been shown to be a potent chemoattractant for monocytes and lymphocytes but not neutrophils. At high concentrations, CCL12 will also chemoattract eosinophils. CCL12 has been found to be a functional ligand for CCR2 but not CCR1, CCR3 or CCR5. |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
| 参考文献 |
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