| 货号 | BAF4497 |
| 别名 | APC2; APOC2; Apo-CII; apoC-II; Apolipoprotein C2; apolipoprotein C-II; MGC75082 |
| 反应种属 | Human |
| 应用 | Western Blot(0.25 µg/mL) |
| 目标/特异性 | Detects recombinant and endogenous human ApoC2 in Western blots. |
| 使用方法 | Western Blot: 0.25 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 344 (Human); 11813 (Mouse); 292697 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human ApoC2 Thr23-Glu101 Accession # P02655 |
| 生物活性 | Human |
| 标记 | Biotin |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Apolipoprotein C2 (ApoC2/II) is a 8‑9 kDa, secreted member of the Apolipoprotein C2 family of proteins. It is produced by hepatocytes and represents a major component of VLDL particles. It activates lipoprotein lipase and may self-associate to form amyloid-type fibrils. The human ApoC2 precursor is 101 amino acids (aa) in length. It contains a 22 amino acid (aa) signal sequence, followed by a 79 aa ProApoC2 that contains a lipid-binding region (aa 43‑51) and an enzyme interaction site (aa 55‑78). ProApoC2 represents > 90% of circulating ApoC2. In human, limited proteolytic processing occurs with removal of the six aa prosegment (aa 23‑28). This does not affect activity. Human ProApoC2 is 59% aa identical to mouse and dog ProApoC2, respectively. |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
| 参考文献 |
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Detection of Human Apolipoprotein C‑II/ApoC2 by Western Blot. Western blot shows lysates of human plasma and small intestine tissue. PVDF membrane was probed with 0.25 µg/mL of Human Apolipoprotein C‑II/ApoC2 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF4497) followed by HRP‑conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Apolipoprotein C-II/ApoC2 at approximately 8 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2. |
