Human Serpin E1/PAI-1 Biotinylated Affinity Purified PAb (50 UG)【科瑞奥博】

产品中心

当前位置：首页>产品中心

Human Serpin E1/PAI-1 Biotinylated Affinity Purified PAb (50 UG)

货号： BAF1786 基本售价： 5958.6 元规格： -

产品信息

概述
货号	BAF1786
描述	For ELISA the Antibody Pairs information：Capture antibody：MAB1786-500；Detection antibody：BAF1786; and protein: 1786-PI-010
别名	Endothelial plasminogen activator inhibitor; Nexin; PAI-1; PAI1PAI-1; PAISerpin E1; PLANH1; PLANH1plasminogen activator inhibitor 1; serine (or cysteine) proteinase inhibitor, clade E (nexin, plasminogenactivator inhibitor type 1), member 1; serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitortype 1), member 1
全称	Plasminogen Activator Inhibitor
反应种属	Human
应用	Western Blot(1 µg/mL) ELISA Capture (Matched Antibody Pair)(2-8 µg/mL ) ELISA Detection (Matched Antibody Pair)(0.1-0.4 µg/mL ) ELISA Standard ( )
目标/特异性	Detects human Serpin E1/PAI-1 in ELISAs and Western blots. In sandwich immunoassays, less than 0.2% cross-reactivity with recombinant human (rh) Serpin A1, rhSerpin A3, rhSerpin A4, rhSerpin A5, and rhSerpin F2 is observed.
使用方法	Western Blot: 1 µg/mL ELISA Capture (Matched Antibody Pair): 2-8 µg/mL ELISA Detection (Matched Antibody Pair): 0.1-0.4 µg/mL ELISA Standard :
来源	Reconstitute at 0.2 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	5054 (Human); 18787 (Mouse)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. A multiplex immunoassay for human adipokine profiling. Authors: Schipper HS, De Jager W, van Dijk ME, Meerding J, Zelissen PM, Adan RA, Prakken BJ, Kalkhoven E Clin. Chem., 2010;56(0):1320. Species: Human Sample Type: Cell Culture Supernates Application: Luminex Development
纯化方式	Antigen Affinity-purified
免疫原	S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Serpin E1/PAI-1 (R&D Systems, Catalog # 1786-PI) Ser22-Pro402 Accession # P05121
生物活性	Human
标记	Biotin
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	As a member of the Serpin superfamily of serine protease inhibitors, Serpin E1/PAI-1 is the principle inhibitor of urokinase-type plasminogen activator (uPA) and tissue-type PA (1, 2). As important regulators of extracellular matrix remodeling, uPA and PAI-1 play a major role in many processes such as angiogenesis, tumor invasion and obesity (2-4). For example, uPA and PAI-1 are the only tumor prognostic factors validated at the highest level of evidence with regard to their clinical utility in breast cancer (5). The human PAI-1 is initially synthesized as 402 amino acid precursor with a N-terminal signal peptide (6, 7). PAI-1 may exist in one of two possible conformations, designated as active or latent (8). The purified rhPAI-1 is active against rhuPA. The heterogeneity at the N-terminus of the purified rhPAI-1 has been observed before for both the recombinant and native proteins (9).
运输条件	Blue Ice
存放说明	-20℃
参考文献	Silverman, G.A. et al. (2001) J. Biol. Chem. 276:33293. Stefansson, S. et al. (2003) Curr. Pharm. Des. 9:1545. Duffy, M.J. (2002) Clin. Chem. 48:1194. Juhan-Vague, I. et al. (2003) J. Thromb. Haemost. 1:1575. Harbeck, N. et al. (2002) Clin. Breast Cancer 3:196. Pannekoek, H. et al. (1986) EMBO J. 5:2539. Ginsburg, D. et al. (1986) J. Clin. Invest. 78:1673. Wang, Z. et al. (1996) Biochemistry 35:16443. Stromqvist, M. et al. (1994) Protein Expr. Purif. 5:309.

参考图片

Detection of Human Serpin E1/PAI‑1 by Western Blot. Western blot shows lysates of HUVEC human umbilical vein endothelial cells. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Serpin E1/PAI‑1 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF1786) followed by Streptavidin-HRP (Catalog # DY998) in 3% BSA. A specific band was detected for Serpin E1/PAI‑1 at approximately 48 kDa (as indicated). This experiment was conducted under reducing conditions.