| 货号 | AB820 |
| 别名 | apoptosis regulator BAX; BCL2-associated X protein; Bcl2-L-4; BCL2L4bcl2-L-4; Bcl-2-like protein 4 | 全称 | Bcl Associated X Protein |
| 反应种属 | Human |
| 应用 | Western Blot(4 µg/mL) Immunohistochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects human Bax Minus C-Terminus. Does not detect mouse Bax. |
| 使用方法 | Western Blot: 4 µg/mL Immunohistochemistry: 5-15 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 581 (Human); 12028 (Mouse); 24887 (Rat) |
| 纯化方式 | Protein A or G purified |
| 免疫原 | Human Bax synthetic peptide |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | B cell leukemia/lymphoma-2 associated X protein (Bax) is a pro-apoptotic member of the Bcl-2 family of proteins found in many cell types. Bax is a cytosolic protein that translocates to the mitochondria and participates in Cytochrome c release in response to apoptotic stimuli. Aberrant expression of Bcl-2 family members can inappropriately promote or prevent apoptosis. Increased expression of Bax is associated with the apoptotic loss of neurons in Parkinsons disease. |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
| 参考文献 |
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Detection of Human Bax Minus C-Terminus by Western Blot. Western blot shows lysates of THP-1 human acute monocytic leukemia cell line. ??? membrane was probed with 4 µg/mL Goat Anti-Human Bax Minus C-Terminus Polyclonal Antibody (Catalog # AB820) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). For additional reference, recombinant ??? Bax (1 ng) was included. A specific band for Bax was detected at approximately ??? kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2. |
