| 货号 | BAF8171 |
| 别名 | AIP1; API2; BIRC3 baculoviral IAP repeat containing 3; BIRC3; CIAP2; c-IAP2; HAIP1; HIAP1; HIAP-1; MALT2; MIHC; RNF49 |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) |
| 目标/特异性 | Detects human cIAP‑2/HIAP‑1 in Western blots. |
| 使用方法 | Western Blot: 1 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 330 (Human) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human cIAP‑2/HIAP‑1 Asn2-Ser604 Accession # U45878 |
| 生物活性 | Human |
| 标记 | Biotin |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | cIAP-2 (also known as MIHC and HIAP-1) is a member of the inhibitor of apoptosis (IAP) family of proteins that inhibit the proteolytic activity of mature caspases. cIAP-2 has 3 BIR (baculovirus inhibitor of apoptosis) domains, a RING finger domain, and a caspase recruitment domain (CARD). cIAP-2 inhibits caspases through the direct interaction of its BIR domain with the active caspase. Caspase activity may be restored through interactions with the Reaper like motif on mitochondrial proteins such as SMAC/Diablo or HtrA2/Omi. cIAP-2 is reported to be cleaved by HtrA2/Omi. |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
| 参考文献 |
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Detection of Human cIAP‑2/HIAP‑1 by Western Blot. Western blot shows lysates of Raji human Burkitts lymphoma cell line and Daudi human Burkitts lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human cIAP‑2/HIAP‑1 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF8171) followed by Streptavidin-HRP (Catalog # DY998). A specific band was detected for cIAP‑2/HIAP‑1 at approximately 68 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
