货号 | BAF559 |
别名 | CNTFR | 全称 | Ciliary Neurotrophic Factor Receptor alpha |
反应种属 | Rat |
应用 | Western Blot(0.1 µg/mL) Immunohistochemistry(5-15 µg/mL) |
目标/特异性 | Detects rat CNTF R alpha in Western blots. |
使用方法 | Western Blot: 0.1 µg/mL Immunohistochemistry: 5-15 µg/mL |
来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
产品组分 |
供应商 | R&D Systems |
Entrez Gene IDs | 1271 (Human); 313173 (Rat) |
纯化方式 | Antigen Affinity-purified |
免疫原 | S. frugiperda insect ovarian cell line Sf 21-derived recombinant rat CNTF R alpha Ala19-Pro346 Accession # Q08406 |
生物活性 | Rat |
标记 | Biotin |
溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
背景 | The high-affinity CNTF receptor complex, which mediates the biological action of CNTF, contains the ligand-binding alpha subunit (CNTF R alpha) and two signal-transducing proteins, LIF R beta and gp130. Whereas LIF R beta and gp130 are widely expressed in many cell types, the expression of CNTF R alpha is restricted to the central and peripheral nervous systems. cDNAs encoding CNTF R alpha isolated from both human and rat share 96% amino acid sequence identity. |
运输条件 | Blue Ice |
存放说明 | -20℃ |
参考文献 |
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CNTF R alpha in Rat Brain. CNTF R alpha was detected in perfusion fixed frozen sections of rat brain using 5 µg/mL Goat Anti-Rat CNTF R alpha Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF559) overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained with the Anti-Goat HRP-AEC Cell & Tissue Staining Kit (red; Catalog # CTS009) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections. |