| 货号 | BAF3689 |
| 描述 | For ELISA the Antibody Pairs information：Capture antibody：MAB3689；Detection antibody：BAF3689; and protein: 3689-TM-050 |
| 别名 | CD365; HAVCR1; HAVCR-1; HAVCRT cell immunoglobin domain and mucin domain protein 1; hepatitis A virus cellular receptor 1; Kidney injury molecule 1; KIM1; KIM-1; T-cell immunoglobulin and mucin domain-containing protein 1; TIM-1TIM; TIM1TIMD-1; TIMD1T-cell membrane protein 1 | 全称 | T Cell Immunoglobulin Mucin-1 |
| 反应种属 | Rat |
| 应用 | Western Blot(0.1 µg/mL) ELISA Capture (Matched Antibody Pair)(2-8 µg/mL ) ELISA Detection (Matched Antibody Pair)(0.1-0.4 µg/mL ) ELISA Standard ( ) |
| 目标/特异性 | Detects rat TIM-1/KIM-1/HAVCR in Western blots. In this format, less than 1% cross-reactivity with recombinant human TIM-1 is observed. |
| 使用方法 | Western Blot: 0.1 µg/mL ELISA Capture (Matched Antibody Pair): 2-8 µg/mL ELISA Detection (Matched Antibody Pair): 0.1-0.4 µg/mL ELISA Standard : |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 26762 (Human); 171283 (Mouse); 286934 (Rat); 102141332 (Cynomolgus Monkey) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant rat TIM-1/KIM-1/HAVCR (R&D Systems, Catalog # 3689-TM) Ser18-Val238 Accession # O54947 |
| 生物活性 | Rat |
| 标记 | Biotin |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | KIM-1 (Kidney-injury molecule-1; also TIM-1 and HAVCR) is a 50 - 80 kDa, variably glycosylated, type I transmembrane glycoprotein that is a member of the TIM family of immunoglobulin superfamily molecules (1 - 5). This gene family is involved in the regulation of Th1 and Th2-cell-mediated immunity. In mouse, there are eight known TIM/KIM genes (# 1 - 8) vs. only three genes in human (# 1, 3, 4) (1, 2, 5). It is unknown if the rat genome exactly parallels that of mouse. Rat KIM-1 is synthesized as a 307 amino acid (aa) precursor that contains a 21 aa signal sequence, a 214 aa extracellular domain (ECD), a 21 aa transmembrane segment and a 51 aa cytoplasmic domain (4). The ECD contains one V-type Ig-like domain and a mucin region characterized by multiple Thr-Ser-Pro motifs. The mucin region may undergo extensive O-linked glycosylation. The mouse KIM-1 gene is highly polymorphic and this may be reflected in rat (4, 6). In human, TIM-1 is known to circulate as a soluble form. It undergoes constitutive cleavage by an undefined MMP, releasing an 85 kDa soluble molecule (7). A similar process has now been described in rat (8). The ECD of rat KIM-1 is 50% and 81% aa identical to human and mouse KIM-1 ECD, respectively. The only two reported ligands for KIM-1 are TIM-4 and the hepatitis A virus (9, 10). However, others are believed to exist, and based on the ligand for TIM-3, one might be an S-type lectin (11). KIM-1 is found on CD4+ T cells and proximal renal tubular cells (4, 12). KIM-1 ligation induces T cell proliferation and promotes cytokine production (1, 11). In particular, it induces IL-4 production, and requires the KIM-1 cytoplasmic tyrosine phosphorylation motif (12). Alternatively, KIM-1 activation of TIM-4 induces cell cycle arrest (13). |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
| 参考文献 |
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