货号 | BAF3250 |
别名 | CD146 antigen; CD146; cell surface glycoprotein MUC18; Cell surface glycoprotein P1H12; L-Gicerin; melanoma adhesion molecule; melanoma cell adhesion moleculeS-endo 1 endothelial-associated antigen; Melanoma-associated antigen A32; Melanoma-associated antigen MUC18; MUC18; MUC18Gicerin | 全称 | Melanoma Cell Adhesion Molecule |
反应种属 | Rat |
应用 | Western Blot(0.1 µg/mL) Flow Cytometry(2.5 µg/106cells) |
目标/特异性 | Detects rat MCAM/CD146 in Western blots. In Western blots, approximately 10% cross-reactivity with recombinant human MCAM is observed. |
使用方法 | Western Blot: 0.1 µg/mL Flow Cytometry: 2.5 µg/106cells |
来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
产品组分 |
供应商 | R&D Systems |
Entrez Gene IDs | 4162 (Human); 84004 (Mouse); 78967 (Rat) |
纯化方式 | Antigen Affinity-purified |
免疫原 | Mouse myeloma cell line NS0-derived recombinant rat MCAM/CD146 Arg19-Lys560 Accession # Q9EPF2 |
生物活性 | Rat |
标记 | Biotin |
溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
背景 | MCAM, also known as CD146 and MUC18, is a type I transmembrane adhesion molecule belonging to the Ig superfamily. It is expressed on all endothelial cells, different epithelial cells, multiple lymphocyte subsets and various tumor cells. Two splice isoforms that differ in their cytoplasmic domains and vasolateral localization have been described. A soluble MCAM is shed and can be detected in circulation. The amino acid sequence of rat MCAM is 72% and 90% identical to that of human and mouse MCAM, respectively. |
运输条件 | Blue Ice |
存放说明 | -20℃ |
参考文献 |
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Detection of MCAM/CD146 in PC‑12 Rat Cell Line by Flow Cytometry. PC‑12 rat adrenal pheochromocytoma cell line was stained with Rat MCAM/CD146 Biotinylated Antigen Affinity‑purified Polyclonal Antibody (Catalog # BAF3250, filled histogram) or isotype control antibody (Catalog # BAF108, open histogram), followed by Streptavidin-Phycoerythrin (Catalog # F0040). |