Canine IFN-gamma Biotinylated Affinity Purified PAb (50 UG)【科瑞奥博】

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Canine IFN-gamma Biotinylated Affinity Purified PAb (50 UG)

货号： BAF781 基本售价： 5532.3 元规格： -

产品信息

概述
货号	BAF781
别名	IFG; IFI; IFNG; IFN-gamma; Immune interferon; interferon gamma; interferon, gamma
全称	Interferon gamma
反应种属	Canine
应用	Western Blot(0.1 µg/mL) Immunocytochemistry(5-15 µg/mL)
目标/特异性	Detects canine IFN-gamma in direct ELISAs and Western blots. In Western blots, approximately 25% cross-reactivity with recombinant human IFN‑ gamma is observed.
使用方法	Western Blot: 0.1 µg/mL Immunocytochemistry: 5-15 µg/mL
来源	Reconstitute at 0.2 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	3458 (Human); 15978 (Mouse); 25712 (Rat); 396991 (Porcine); 281237 (Bovine); 403801 (Canine); 493965 (Feline)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Long-lasting protection against canine visceral leishmaniasis using the LiESAp-MDP vaccine in endemic areas of France: double-blind randomised efficacy field trial. Authors: Lemesre JL, Holzmuller P, Goncalves RB, Bourdoiseau G, Hugnet C, Cavaleyra M, Papierok G Vaccine, 2007;25(21):4223-34. Species: Canine Sample Type: Cell Culture Supernates Application: ELISA Development Vaccination with irradiated Ancylostoma caninum third stage larvae induces a Th2 protective response in dogs. Authors: Fujiwara RT, Loukas A, Mendez S, Williamson AL, Bueno LL, Wang Y, Samuel A, Zhan B, Bottazzi ME, Hotez PJ, Bethony JM Vaccine, 2005;24(4):501-9. Species: Canine Sample Type: Cell Culture Supernates Application: ELISA Development Interleukin-12 augments a Th1-type immune response manifested as lymphocyte proliferation and interferon gamma production in Leishmania infantum-infected dogs. Authors: Strauss-Ayali D, Baneth G, Shor S, Okano F, Jaffe CL Int. J. Parasitol., 2005;35(1):63-73. Species: Canine Sample Type: Cell Culture Supernates Application: ELISA Development Protection against experimental visceral leishmaniasis infection in dogs immunized with purified excreted secreted antigens of Leishmania infantum promastigotes. Authors: Lemesre JL, Holzmuller P, Cavaleyra M, Goncalves RB, Hottin G, Papierok G Vaccine, 2005;23(22):2825-40. Species: Canine Sample Type: Cell Culture Supernates Application: ELISA Development
纯化方式	Antigen Affinity-purified
免疫原	E. coli-derived recombinant canine IFN-gamma Gln24-Lys166 Accession # P42161
生物活性	Canine
标记	Biotin
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	Interferon-gamma (IFN-gamma ), also known as type II or immune interferon, exerts a wide range of immunoregulatory activities and is considered to be the prototype proinflammatory cytokine (1, 2). Mature canine IFN-gamma exists as a noncovalently linked homodimer of 20-25 kDa variably glycosylated subunits (3). It shares 79-88% amino acid sequence identity with bovine, equine, and feline IFN-gamma, 62-73% with human, porcine, and rhesus IFN-gamma, and 40-47% with cotton rat, mouse, and rat IFN-gamma. IFN-gamma dimers bind to IFN-gamma RI (alpha subunits) which then interact with IFN-gamma RII (beta subunits) to form the functional receptor complex of two alpha and two beta subunits. Inclusion of IFN-gamma RII increases the binding affinity for ligand and the efficiency of signal transduction (4, 5). IFN-gamma is produced by a variety of immune cells under inflammatory conditions, notably by T cells and NK cells (6). It plays a key role in host defense by promoting the development and activation of Th1 cells, chemoattraction and activation of monocytes and macrophages, upregulation of antigen presentation molecules, and immunoglobulin class switching in B cells. It also exhibits antiviral, antiproliferative, and apoptotic effects (6, 7). In addition, IFN-gamma functions as an anti-inflammatory mediator by promoting the development of regulatory T cells and inhibiting Th17 cell differentiation (8, 9). The pleiotropic effects of IFN-gamma contribute to the development of multiple aspects of atherosclerosis (7).
运输条件	Blue Ice
存放说明	-20℃
参考文献	Billiau, A. and P. Matthys (2009) Cytokine Growth Factor Rev. 20:97. Pestka, S. et al. (2004) Immunol. Rev. 202:8. Zucker, K. et al. (1992) J. Interferon Res. 12:191. Marsters, S.A. et al. (1995) Proc. Natl. Acad. Sci. USA 92:5401. Krause, C.D. et al. (2000) J. Biol. Chem. 275:22995. Schroder, K. et al. (2004) J. Leukoc. Biol. 75:163. McLaren, J.E. and D.P. Ramji (2009) Cytokine Growth Factor Rev. 20:125. Muhl, H. and J. Pfeilschifter (2003) Int. Immunopharmacol. 3:1247. Kelchtermans, H. et al. (2008) Trends Immunol. 29:479.

参考图片

IGFBP‑1 in Canine PBMCs. IGFBP‑1 was detected in immersion fixed canine peripheral blood mononuclear cells (PBMCs) stimulated with PMA and calcium ionomycin using Goat Anti-Human IGFBP‑1 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF871) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (red; Catalog # NL999) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.