| 货号 | BAF565 |
| 描述 | For ELISA the Antibody Pairs information：Capture antibody：AF565；Detection antibody：BAF565; and protein: 565-RG-025 |
| 别名 | IFG; IFI; IFNG; IFN-gamma; Immune interferon; interferon gamma; interferon, gamma | 全称 | Interferon gamma |
| 应用 | Western Blot(0.1 µg/mL) ELISA Capture (Matched Antibody Pair)(0.2-0.8 µg/mL ) ELISA Detection (Matched Antibody Pair)(0.1-0.4 µg/mL ) ELISA Standard ( ) |
| 目标/特异性 | Detects cotton rat IFN-gamma in ELISAs and Western blots. In sandwich immunoassays, less than 0.3% cross‑reactivity with recombinant human (rh) IFN‑ gamma, recombinant mouse (rm) IFN‑ gamma, recombinant rat (rr) IFN‑ gamma, recombinant porcine (rp) IFN‑ gamma, recombinant canine IFN‑ gamma, and recombinant feline (rfe) IFN‑ gamma is observed. |
| 使用方法 | Western Blot: 0.1 µg/mL ELISA Capture (Matched Antibody Pair): 0.2-0.8 µg/mL ELISA Detection (Matched Antibody Pair): 0.1-0.4 µg/mL ELISA Standard : |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 3458 (Human); 15978 (Mouse); 25712 (Rat); 396991 (Porcine); 281237 (Bovine); 403801 (Canine); 493965 (Feline) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Role of AKT kinase in measles virus replication. | |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant cotton rat IFN-gamma His21-Ile170 Accession # Q9QXX2 |
| 生物活性 | Cotton Rat |
| 标记 | Biotin |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Interferon-gamma (IFN-gamma ), also known as type II or immune interferon, exerts a wide range of immunoregulatory activities and is considered to be the prototype proinflammatory cytokine (1, 2). Mature cotton rat IFN-gamma exists as a noncovalently linked homodimer of 20‑25 kDa variably glycosylated subunits (3). It shares 43%‑50% amino acid sequence identity with bovine, canine, equine, feline, human, mouse, rat, rhesus, and porcine IFN-gamma. IFN-gamma dimers bind to IFN-gamma RI (alpha subunits) which then interact with IFN-gamma RII (beta subunits) to form the functional receptor complex of two alpha and two beta subunits. Inclusion of IFN-gamma RII increases the binding affinity for ligand and the efficiency of signal transduction (4, 5). IFN-gamma is produced by a variety of immune cells under inflammatory conditions, notably by T cells and NK cells (6). It plays a key role in host defense by promoting the development and activation of Th1 cells, chemoattraction and activation of monocytes and macrophages, upregulation of antigen presentation molecules, and immunoglobulin class switching in B cells. It also exhibits antiviral, antiproliferative, and apoptotic effects (6, 7). In addition, IFN-gamma functions as an anti-inflammatory mediator by promoting the development of regulatory T cells and inhibiting Th17 cell differentiation (8, 9). The pleiotropic effects of IFN-gamma contribute to the development of multiple aspects of atherosclerosis (7). |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
| 参考文献 |
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