| 货号 | BAF1082 |
| 别名 | IFNalpha; IFN-alpha | 全称 | Interferon alpha |
| 应用 | Western Blot(0.1 µg/mL) |
| 目标/特异性 | Detects cotton rat IFN-alpha in Western blots. In this format, less than 1% cross-reactivity with recombinant human IFN-beta and recombinant cotton rat IFN-gamma is observed. |
| 使用方法 | Western Blot: 0.1 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 3440 (Human) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant cotton rat IFN-alpha (R&D Systems, Catalog # 1082-CR) Cys24-Lys190 with a Cys109Tyr substitution Accession # AAL18816 |
| 生物活性 | Cotton Rat |
| 标记 | Biotin |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Interferon-alpha, also known as leukocyte interferon, comprises a group of related but distinct proteins that share over 95% amino acid sequence homology. They are members of the type I interferon family which share a common cell surface receptor composed of two subunits, a 100 kDa ligand-binding subunit (IFN-alpha R1) and a 125 kDa subunit (IFN-alpha R2) that is involved both in ligand-binding and signal transduction. IFN-alpha is expressed primarily by leukocytes upon activation by viruses, bacteria, cytokines and growth factors. IFN-alpha has been shown to have anti-viral and immunomodulatory activities on target cells. It can also inhibit cell proliferation as well as induce apoptosis (1, 2). Cotton rat IFN-alpha 1 cDNA encodes a 189 amino acid (aa) residue precursor protein with a putative 23 aa signal sequence and a 166 aa mature protein. The protein contains five cysteines, four of which are involved in two intrachain disulfide bonds. Cotton rat IFN-alpha 1 shares 52%, 67%, 68% and 72% aa sequence identity to human, rat, hamster and mouse IFN-alpha 1, respectively. |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
| 参考文献 |
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