| 货号 | BAF958 |
| 别名 | GDF-3; growth differentiation factor 3; growth/differentiation factor 3; KFS3; MCOP7; MCOPCB6; Vgr-2 | 全称 | Growth Differentiation Factor 3 |
| 反应种属 | Mouse |
| 应用 | Western Blot(0.1 µg/mL) |
| 目标/特异性 | Detects mouse GDF-3 in Western blots. In Western blots, approximately 20% cross‑reactivity with recombinant mouse (rm) GDF-1 is observed and less than 5% cross-reactivity with rmGDF-5, rmGDF-6, rmGDF-8 and rmGDF-9 is observed. |
| 使用方法 | Western Blot: 0.1 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 9573 (Human); 14562 (Mouse) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant mouse GDF-3 Ile255-Gly366 Accession # Q07104 |
| 生物活性 | Mouse |
| 标记 | Biotin |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | GDF-3 (previously called Vgr-2) is a TGF-beta superfamily member belonging to the growth/differentiation factor family (1, 2). GDF-3 is expressed in undifferentiated embryonic stem (ES) cells, adipose tissue and the brain (2-4). In ES cells, it maintains pluripotency and influences early cell fate decisions (5, 6). For example, frog embryos injected with GDF-3 develop a secondary dorsal axis and deletion of mouse GDF-3 can produce defects in the anterior visceral endoderm of the pre‑gastrulation embryo (5, 6). In adipocytes, GDF-3 is induced by a high fat diet and promotes adipogenesis (3). GDF-3 has been reported to oppose BMP’s functions and to have a nodal-like activity in early development (1). The 366 amino acid (aa) mouse GDF-3 contains a 22 aa signal sequence, a 230 aa propeptide and a 114 aa mature protein that contains one potential N-glycosylation site. Most of GDF-3 is present as the prepro form, while the mature GDF-3 is presumably the secreted, active form (1). The mature protein contains the cysteine-knot structure that is conserved throughout family members. Since it lacks the fourth cysteine, which is responsible for the formation of inter-molecular disulfide bond, GDF-3 may exist as a non-covalent homodimer. Mature mouse GDF-3 shares 90%, 83%, and 83% aa identity with rat, human and canine GDF-3, respectively. Among family members, mature GDF-3 is most similar to mouse BMP-6 (45% aa identity) and Xenopus VG‑1 (52% aa identity). |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
| 参考文献 |
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