| 货号 | BAF749 |
| 别名 | LT; LTA; LT-alpha; lymphotoxin alpha (TNF superfamily, member 1); Lymphotoxin alpha; tnfb; TNF-beta; TNFBlymphotoxin-alpha; TNFSF1; TNFSF1B; TNFSF1TNF-beta; tumor necrosis factor beta; Tumor necrosis factor ligand superfamily member 1 | 全称 | Tumor Necrosis Factor beta |
| 反应种属 | Mouse |
| 应用 | Western Blot(0.1 µg/mL) Immunohistochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects mouse Lymphotoxin‑ alpha /TNF‑ beta in Western blots. In Western blots, approximately 5% cross-reactivity with recombinant human Lymphotoxin‑ alpha /TNF‑ beta is observed (non-reducing conditions). |
| 使用方法 | Western Blot: 0.1 µg/mL Immunohistochemistry: 5-15 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 4049 (Human); 16992 (Mouse) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant mouse Lymphotoxin‑ alpha /TNF‑ beta |
| 生物活性 | Mouse |
| 标记 | Biotin |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Tumor necrosis factor-beta (TNF-beta ), also known as lymphotoxin-alpha (LT-alpha ), is a secreted homotrimeric glycoprotein belonging to the TNF superfamily and is designated TNFSF1B. It is produced by NK, T, and B cells. TNF-beta was originally identified as protein that kills tumor cells in cell culture supernatants of a lymphoblastoid cell line. The TNF-beta subunit also associates with the type II transmembrane TNF superfamily protein lymphotoxin beta (LT beta ) to generate two types of heterotrimers designated as LT alpha 1 beta 2 (a single TNF-beta chain non-covalently associated with two chains of LT beta ), and LT alpha 2 beta 1 (1, 2). TNF-alpha, TNF-beta, and LT beta form a subfamily of the TNF related ligands. Their genes are genetically linked within a compact cluster inside the major histocompatibility complex locus (2, 3). The soluble TNF-beta binds and signals through TNF R1 and TNF R2. In contrast, the membrane-bound LT alpha 1 beta 2 interacts specifically with the LT beta receptor (LT beta R), which does not bind TNF-beta or TNF-alpha. Both TNFR1 and TNFR2 bind LT alpha 2 beta 1, which is recognized weakly by LT beta R (4, 5). TNF R1 and 2 express very broadly, while expression of LT beta R is restricted to stromal cells of lymphoid tissues. Herpesvirus entry mediator binds TNF-beta in vitro (6). The physiological importance of such interaction, if it occurs in vivo, is unclear. Distinct functions attributed to TNF-beta from transgenic knock-out mice include, loss of lymph node development, change in splenic architecture, impaired germinal center formation, and susceptibility to pulmonary tuberculosis (7, 8). TNF-beta also has overlapping physiological functions with LT beta and TNF-alpha in lymphoid organogenesis (7). Mouse and human TNF-beta share approximately 74% homology in their amino acid sequence. |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
| 参考文献 |
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