| 货号 | BAF472 |
| 描述 | For ELISA the Antibody Pairs information：Capture antibody：MAB571；Detection antibody：BAF472; and protein: 472-FF-025 |
| 别名 | ABCD-3; C3Xkine; chemokine (C-X3-C motif) ligand 1; CX3C membrane-anchored chemokine; CXC3; CXC3C; FKN; Fractalkine; Neurotactin; neurotactin); NTNsmall inducible cytokine subfamily D (Cys-X3-Cys), member 1 (fractalkine; NTTSmall-inducible cytokine D1; SCYD1C-X3-C motif chemokine 1; small inducible cytokine subfamily D (Cys-X3-Cys), member-1 |
| 反应种属 | Mouse |
| 应用 | Western Blot(0.1 µg/mL) ELISA Capture (Matched Antibody Pair)(2-8 µg/mL ) ELISA Detection (Matched Antibody Pair)(0.1-0.4 µg/mL ) ELISA Standard ( ) |
| 目标/特异性 | Detects mouse CX3CL1/Fractalkine in ELISAs and Western blots. In sandwich immunoassays, less than 2% cross‑reactivity with recombinant human (rh) Fractalkine (chemokine domain) is observed, less than 0.3% cross‑reactivity with rhFractalkine (full length) is observed and less than 0.1% cross‑reactivity with recombinant rat Fractalkine (full length and chemokine domain) is observed. |
| 使用方法 | Western Blot: 0.1 µg/mL ELISA Capture (Matched Antibody Pair): 2-8 µg/mL ELISA Detection (Matched Antibody Pair): 0.1-0.4 µg/mL ELISA Standard : |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 6376 (Human); 20312 (Mouse); 89808 (Rat) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Effect of fractalkine-Fc delivery in experimental lung metastasis using DNA/704 nanospheres. | |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant mouse CX3CL1/Fractalkine (R&D Systems, Catalog # 458-MF) Leu22-Lys105 Accession # O35188 |
| 生物活性 | Mouse |
| 标记 | Biotin |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | CX3CL1, also named neurotactin, is a member of the delta chemokine subfamily that contains a novel C-X3-C motif. Unlike other known chemokines, CX3CL1 is a type 1 membrane protein containing a chemokine domain tethered on a long mucin-like stalk. Mouse CX3CL1 cDNA encodes a 395 amino acid (aa) residue precursor protein with a predicted 24 or an alternate 21 aa residue signal peptide, a 76 aa residue globular chemokine domain, a 236 aa residue stalk region rich in Gly, Pro, Ser and Thr and containing degenerate mucin-like repeats, a 21 aa residue transmembrane segment and a 38 aa residue cytoplasmic domain. The extracellular domain of mouse and human CX3CL1 can potentially be released as a soluble protein by proteolysis at the conserved dibasic motif proximal to the transmembrane region. With the exception of the stalk region, human and mouse CX3CL1 share a high degree of amino acid sequence homology (> 75% sequence identity). CX3CL1 is expressed in various tissues including heart, brain, lung, kidney, skeletal muscle, and testis. In rat brain, CX3CL1 expression was found to be localized principally to neurons. The expression of CX3CL1 was also reported to be up-regulated on activated endothelial cells. Membrane-bound CX3CL1 has been shown to promote adhesion of leukocytes. The soluble chemokine domain of human CX3CL1 was reported to be chemotactic for T cells and monocytes while the soluble chemokine domain of mouse CX3CL1 was reported to chemoattract neutrophils and T-lymphocytes but not monocytes. The gene for mouse CX3CL1 has been mapped to chromosome 11. CX3CR1, previously named V28 or chemokine beta receptor-like 1, has been found to be a specific receptor for CX3CL1. In addition, US28, a 7TM receptor encoded by human cytomegalovirus that binds multiple CC chemokines, has also been shown to bind CX3CL1 with high-affinity. |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
| 参考文献 |
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