| 货号 | BAF4636 |
| 别名 | AMY; AMYolfactomedin related ER localized protein; neuroblastoma protein; Neuronal olfactomedin-related ER localized protein; NOE1; NOE1noelin; NOEL1; NOELIN; NOELIN1; Noelin-1; OlfA; olfactomedin 1; olfactomedin-1; OLFM1; pancortin 1 |
| 反应种属 | Mouse |
| 应用 | Western Blot(0.1 µg/mL) |
| 目标/特异性 | Detects mouse Olfactomedin-1/Noelin-1 in Western blots. |
| 使用方法 | Western Blot: 0.1 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 10439 (Human) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant mouse Olfactomedin‑1/Noelin‑1 Ser2-Arg481 Accession # NP_062371 |
| 生物活性 | Mouse |
| 标记 | Biotin |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Olfactomedin-1, also known as Noelin-1 (neuronal olfactomedin-related endoplasmic reticulum-localized-1), or pancortin, is a 75 kDa secreted extracellular matrix glycoprotein expressed in the brain. Alternate promoter usage and splicing creates four forms of Noelin (Noelin 1-4) that are combinations of a common central region with alternate N- and C-termini. Olfactomedin-1/Noelin-1 is the longest of the four isoforms, encoding a 16 amino acid (aa) signal sequence and a 469 aa mature protein with a coiled-coil region for multimerization and an olfactomedin-like domain. The shortest isoforms, Noelin-3 and -4, are truncated within the coiled-coil region and lack the olfactomedin-like domain. In the species tested, these isoforms appear to oppose actions of the longer isoforms. All isoforms are capable of forming homo- or heterodimers through cysteines in their common central region. The C-terminus of Olfactomedin-1 (mouse aa 482-485) contains a putative endoplasmic reticulum retention sequence that has not been included in the R&D Systems protein. Mouse Olfactomedin-1 (aa 17-481) shares 99%, 99%, 97%, 96% and 93% aa identity with corresponding regions of rat, human, opossum, chicken and Xenopus Olfactomedin-1, respectively. In vivo expression patterns differ somewhat between species. In chick early development, Olfactomedin-1 shows highest concentration in areas that produce neural crest cells and is produced by migrating neural crest cells. Overproduction of Olfactomedin-1 prolongs neural crest cell formation. Olfactomedin-1 is also expressed in mouse neural crest, but not frog. All three species express Olfactomedin-1 later in differentiating neural tissues in the spinal cord, brain and cranial ganglia. |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |