Mouse Osteoprotegerin/TNFRSF11B Biotin Affinity Purified PAb (50 UG)【科瑞奥博】

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Mouse Osteoprotegerin/TNFRSF11B Biotin Affinity Purified PAb (50 UG)

货号： BAF459 基本售价： 5532.3 元规格： -

产品信息

概述
货号	BAF459
描述	For ELISA the Antibody Pairs information：Capture antibody：MAB4591；Detection antibody：BAF459; and protein: 459-MO-100
别名	OCIF; OCIFMGC29565; OPGtumor necrosis factor receptor superfamily member 11B; Osteoclastogenesis inhibitory factor; Osteoprotegerin; TNFRSF11B; TR1; tumor necrosis factor receptor superfamily, member 11b
反应种属	Mouse
应用	Western Blot(0.1 µg/mL) Immunohistochemistry(5-15 µg/mL) ELISA Capture (Matched Antibody Pair)(2-8 µg/mL ) ELISA Detection (Matched Antibody Pair)(0.1-0.4 µg/mL ) ELISA Standard ( )
目标/特异性	Detects mouse Osteoprotegerin/TNFRSF11B in ELISAs and Western blots. In sandwich immunoassays, less than 0.2% cross-reactivity with recombinant mouse RANK is observed.
使用方法	Western Blot: 0.1 µg/mL Immunohistochemistry: 5-15 µg/mL ELISA Capture (Matched Antibody Pair): 2-8 µg/mL ELISA Detection (Matched Antibody Pair): 0.1-0.4 µg/mL ELISA Standard :
来源	Reconstitute at 0.2 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	4982 (Human); 18383 (Mouse)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Osteoprotegerin-Mediated Homeostasis of Rank+ Thymic Epithelial Cells Does Not Limit Foxp3+ Regulatory T Cell Development. Authors: McCarthy N, Cowan J, Nakamura K, Bacon A, Baik S, White A, Parnell S, Jenkinson E, Jenkinson W, Anderson G J Immunol, 2015;195(6):2675-82. Species: Mouse Sample Type: Whole Cells Application: Flow OPG/RANKL/RANK axis is a critical inflammatory signaling system in ischemic brain in mice. Authors: Shimamura M, Nakagami H, Osako M, Kurinami H, Koriyama H, Zhengda P, Tomioka H, Tenma A, Wakayama K, Morishita R Proc Natl Acad Sci U S A, 2014;111(22):8191-6. Species: Mouse Sample Type: Whole Tissue Application: IHC Fluorescence Cytokine-induced osteoprotegerin expression protects pancreatic beta cells through p38 mitogen-activated protein kinase signalling against cell death. Authors: Schrader J, Rennekamp W, Niebergall U, Schoppet M, Jahr H, Brendel MD, Horsch D, Hofbauer LC Diabetologia, 2007;50(6):1243-7. Species: Rat Sample Type: Cell Culture Supernates Application: ELISA Development Therapeutic relevance of osteoprotegerin gene therapy in osteosarcoma: blockade of the vicious cycle between tumor cell proliferation and bone resorption. Authors: Lamoureux F, Richard P, Wittrant Y, Battaglia S, Pilet P, Trichet V, Blanchard F, Gouin F, Pitard B, Heymann D, Redini F Cancer Res., 2007;67(15):7308-18. Species: Mouse Sample Type: Whole Tissue Application: IHC Paraffin-embedded Serotonin and fluoxetine modulate bone cell function in vitro. Authors: Gustafsson BI, Thommesen L, Stunes AK, Tommeras K, Westbroek I, Waldum HL, Slordahl K, Tamburstuen MV, Reseland JE, Syversen U J. Cell. Biochem., 2006;98(1):139-51. Species: Mouse Sample Type: Cell Culture Supernates Application: ELISA Development 17beta-estradiol (E2) modulates cytokine and chemokine expression in human monocyte-derived dendritic cells. Authors: Bengtsson AK, Ryan EJ, Giordano D, Magaletti DM, Clark EA Blood, 2004;104(5):1404-10. Species: Human Sample Type: Cell Culture Supernates Application: ELISA Development
纯化方式	Antigen Affinity-purified
免疫原	Mouse myeloma cell line NS0-derived recombinant mouse Osteoprotegerin/TNFRSF11B Gln21-Leu401 (Gln138Arg) Accession # Q6PI12
生物活性	Mouse
标记	Biotin
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	Osteoprotegerin (OPG)/Osteoclastogenesis Inhibitory Factor (OCIF) is member of the tumor necrosis factor receptor superfamily that lacks any apparent cell‑association motifs and exists as a soluble secreted protein. In the new TNF superfamily nomenclature, OPG is referred to as TNFRSF11B. OPG was originally isolated by sequence homology as a TNF receptor family protein during a fetal rat intestine cDNA-sequencing project and subsequently shown to be involved in the regulation of bone density. OCIF was initially purified from the conditioned medium of human embryonic fibroblasts based on its ability to inhibit osteoclast development. Comparison of the amino-acid sequences of human OPG and OCIF proteins revealed their identity. The amino-terminal half of OPG contains four cysteine-rich repeats characteristic of TNF receptor family members. The carboxy-terminal of OPG/OCIF was found to contain two death domain homologous regions in tandem. Human and mouse OPG share approximately 84% and 94% amino acid sequence identity, respectively, with the rat OPG. Natural OPG/OCIF has been found to exist predominantly as disulfide-linked dimers. Two TNF superfamily ligands, including the membrane proteins OPG ligand/TRANCE (tumor necrosis factor‑related activation-induced cytokine)/ODF (osteoclast differentiation factor)/RANKL (receptor activator of NF-kappaB ligand) and TRAIL (TNF-related apoptosis‑inducing ligand)/APO-2 ligand, have been shown to be the cellular ligands for OPG/OCIF. Each of these ligands has been shown to interact with additional TNF receptor family members, including RANK (with TRANCE) and TRAIL receptors 1‑4 (with TRAIL). The roles of these receptor-ligands in osteoclastogenesis, apoptosis and in the immune system remains to be elucidated.
运输条件	Blue Ice
存放说明	-20℃
参考文献	Lacey, D.L. et al. (1998) Cell 93:165. Emery, J.G. et al. (1998) J. Biol. Chem. 273:14363. Yasuda, H. et al. (1998) Proc. Natl. Acad. Sci. USA 95:3597.

参考图片

Osteoprotegerin/TNFRSF11B in Mouse Intestine. Osteoprotegerin/TNFRSF11B was detected in perfusion fixed frozen sections of mouse intestine using Goat Anti-Mouse Osteoprotegerin/TNFRSF11B Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF459) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.