| 货号 | BAF3117 |
| 别名 | CD316 antigen; CD316; CD81P3; CD81P3LIR-D1; EWI2; EWI-2; EWI2CD81 partner 3; Glu-Trp-Ile EWI motif-containing protein 2; IgSF8; immunoglobulin superfamily, member 8; KCT4; KCT-4; Keratinocytes-associated transmembrane protein 4; PGRL; PGRLimmunoglobulin superfamily member 8 | 全称 | Immunoglobulin Superfamily, Member 8 |
| 反应种属 | Mouse |
| 应用 | Western Blot(0.1 µg/mL) Flow Cytometry(0.25 µg/106cells) Immunocytochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects mouse IGSF8/CD316 in Western blots. In Western blots, approximately 10% cross-reactivity with recombinant human IGSF8 is observed and less than 1% cross-reactivity with recombinant mouse IGSF4 is observed. |
| 使用方法 | Western Blot: 0.1 µg/mL Flow Cytometry: 0.25 µg/106cells Immunocytochemistry: 5-15 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 93185 (Human) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant mouse IGSF8/CD316 Ala25-Thr577 Accession # NP_536344 |
| 生物活性 | Mouse |
| 标记 | Biotin |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | IGSF8, also known as PGRL (PG regulatory-like protein), KASP (KAI/CD82associated protein) and EWI-2 (Glu-Trp-Ile motif 2), is a widelyexpressed transmembrane adhesion protein. It interacts with beta 1Integrins and various tetraspanins including CD9, CD81 and CD82. IGSF8contains four extracellular Ig-like domains. IGSF8 overexpression intransformed cells inhibits cell migration and suppresses cancermetastatic potential. Mouse and human IGSF8 share 90% amino acidsequence identity. |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
| 参考文献 |
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IGSF8/CD316 in Neuro‑2A Mouse Cell Line. IGSF8/CD316 was detected in immersion fixed Neuro‑2A mouse neuroblastoma cell line using Goat Anti-Mouse IGSF8/CD316 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF3117) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (red; Catalog # NL999) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Cells on Coverslips. |
