| 货号 | BAF3059 |
| 别名 | 60B8AG; CAGACP-10; Calgranulin A; calgranulin-A; Calprotectin L1L subunit; CFAG; CFAGL1Ag; CGLA; Cystic fibrosis antigen; Leukocyte L1 complex light chain; MA387; MIF; Migration inhibitory factor-related protein 8; MRP8; MRP-8; MRP8S100 calcium binding protein A8 (calgranulin A); NIF; P8; protein S100-A8; S100 calcium binding protein A8; S100 calcium-binding protein A8 (calgranulin A); S100 calcium-binding protein A8; Urinary stone protein band A | 全称 | S100 Calcium Binding Protein A8 |
| 反应种属 | Mouse |
| 应用 | Western Blot(0.1 µg/mL) Immunocytochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects mouse S100A8 in Western blots. In Western blots, less than 1% cross-reactivity with recombinant mouse (rm) S100A9, rmS100A10, recombinant human (rh) S100B, and rhS100P is observed. |
| 使用方法 | Western Blot: 0.1 µg/mL Immunocytochemistry: 5-15 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 6279 (Human); 20201 (Mouse) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Complicated urinary tract infection is associated with uroepithelial expression of proinflammatory protein S100A8. | |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant mouse S100A8 Pro2-Glu89 Accession # P27005 |
| 生物活性 | Mouse |
| 标记 | Biotin |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Mouse S100A8, also known as CP-10, Calgranulin A and MRP8, is a 10 kDamember of the S100 family of calcium-binding proteins. S100A8 containsshort sequential modules, including an N-terminal alpha -helix, a Ca++-bindingEF-hand segment, a short central linker region, a second EF-handsegment, and a C-terminal alpha -helix. S100A8 noncovalently heterodimerizeswith S100A9. In the presence of Ca++, the heterodimers formheterotetramers. The dimeric complex is found both intracellularly andextracellularly. It binds to heparan sulfate and is chemotactic forPMNs and macrophages. The amino acid sequence of mouse S100A8 is 80%and 57% identical to that of rat and human S100A8, respectively. |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
| 参考文献 |
|
S100A8 in NMuMG Mouse Cell Line. S100A8 was detected in immersion fixed NMuMG mouse mammary gland epithelial cell line using 10 µg/mL Goat Anti-Mouse S100A8 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF3059) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Streptavidin (red; Catalog # NL999) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips. |
