| 货号 | BAF2749 |
| 别名 | APC receptor; CCCA; CCD41; CCD41centrosome-associated protein; CD201 antigen; CD201; centrocyclin; Endothelial cell protein C receptor; endothelial protein C receptor; EPCRMGC23024; PROCR; protein C receptor, endothelial | 全称 | Endothelial Protein C Receptor |
| 反应种属 | Mouse |
| 应用 | Western Blot(0.1 µg/mL) Flow Cytometry(2.5 µg/106cells) Immunocytochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects mouse EPCR in Western blots. In Western blots, approximately 10% cross-reactivity with recombinant human EPCR is observed. |
| 使用方法 | Western Blot: 0.1 µg/mL Flow Cytometry: 2.5 µg/106cells Immunocytochemistry: 5-15 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 10544 (Human); 19124 (Mouse) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant mouse EPCR Ala17-Ser214 Accession # Q64695 |
| 生物活性 | Mouse |
| 标记 | Biotin |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Protein C is a vitamin K-dependent serine protease that plays a major role in blood coagulation. Binding of Protein C to EPCR (CD201) leads to the proteolytic activation of PAR1 (protease-activated receptor 1) on endothelial cells and subsequent up-regulation of Protein C-induced genes. EPCR is a type I transmembrane glycoprotein in the CD1/MHC family. It is expressed most strongly in the endothelial cells of arteries and veins in heart and lung. Membrane bound EPCR is released by metalloproteolytic cleavage to generate the soluble receptor. The extracellular domain of human and mouse EPCR shares approximately 61% amino acid sequence homology. |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
| 参考文献 |
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Detection of EPCR in bEnd.3 Mouse Cell Line by Flow Cytometry. bEnd.3 mouse endothelioma cell line was stained with Goat Anti-Mouse EPCR Biotinylated Antigen Affinity‑purified Polyclonal Antibody (Catalog # BAF2749, filled histogram) or control antibody (Catalog # BAF108, open histogram), followed by Streptavidin-Allophycocyanin (Catalog # F0050). |
