| 货号 | BAF2154 |
| 别名 | B7h.5; B7-H4; B7H4T-cell costimulatory molecule B7x; B7S1; B7S1VCTN1; B7x; B7XPRO1291; FLJ22418; Immune costimulatory protein B7-H4; Protein B7S1; T cell costimulatory molecule B7x; V-set domain containing T cell activation inhibitor 1; V-set domain-containing T-cell activation inhibitor 1; Vtcn1 | 全称 | B7 Homolog 4 |
| 反应种属 | Mouse |
| 应用 | Western Blot(0.1 µg/mL) Flow Cytometry(2.5 µg/106cells) |
| 目标/特异性 | Detects mouse B7-H4 in Western blots. In Western blots, less than 1% cross-reactivity with recombinant mouse (rm) B7‑1, rmB7‑2, rmB7‑H1, rmB7‑H2, rmB7‑H3, and rmPD‑L2 is observed. |
| 使用方法 | Western Blot: 0.1 µg/mL Flow Cytometry: 2.5 µg/106cells |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 79679 (Human); 242122 (Mouse) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Tissue-expressed B7x affects the immune response to and outcome of lethal pulmonary infection. | |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant mouse B7-H4 Phe29-Pro258 Accession # Q7TSP5 |
| 生物活性 | Mouse |
| 标记 | Biotin |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | B7-H4, also known as B7x and B7S1, is a member of the B7 family of immune co-stimulatory proteins (1). Mature B7-H4 is a 50 kDa - 80 kDa glycosylated molecule with a 28 kDa protein core (2). Partial sensitivity to cleavage by PI-PLC suggests a possible GPI linkage of mouse B7-H4 to the cell membrane (3). The 230 amino acid (aa) extracellular region of B7-H4 contains one Ig-like V-set domain and one Ig-like C2-set domain which is followed by a hydrophobic C-terminal region (3 - 5). Within the ECD, mouse B7-H4 shares 90% and 99% aa sequence identity with human and rat B7-H4, respectively. It shares 21% - 29% aa sequence identity with B7-1, B7-2, B7-H1, B7-H2, B7-H3, and PD-L2. B7-H4 expression is induced on mitogen- or LPS-activated B cells, T cells, dendritic cells, monocytes, and macrophages (3, 4) and blocked by GM-CSF or IL-4 (6). It is also expressed on various normal epithelia and upregulated in several carcinomas and renal tubule epithelial cell lesions (2, 6 - 10). B7-H4 is expressed on the surface of macrophages but intracellularly in ovarian and breast cancer cells (2, 6 - 7). It is found in the serum and ascites fluid of cancer patients (11). B7-H4 binds an unidentified ligand on activated T cells which is distinct from BTLA, CD28, CTLA4, ICOS, and PD-1 (3 - 5, 12). Exposure to B7-H4 inhibits antigen-dependent induction of T cell proliferation and activation (3 - 5). Alternatively, B7-H4 expressing renal tubular epithelial cells promote T cell activation (8). Regulatory T cells, IL-6, and IL-10 induce B7-H4 expression on antigen presenting cells which fosters tumor growth by dampening the anti-tumor immune response (6, 13). B7-H4 also promotes the malignant transformation of epithelial cells by protecting them from apoptosis (2). Despite evidence for the involvement of B7-H4 in immune regulation, mice deficient in its expression do not show significant immune deficiencies, suggesting compensation by other molecules in vivo(14). |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
| 参考文献 |
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