货号 | BAF1566 |
别名 | GH1; GHIGHD1B; GHNGrowth hormone; GH-Nsomatotropin; growth hormone 1Pituitary growth hormone; hGH-N; Somatotropin |
反应种属 | Mouse/Rat |
应用 | Western Blot(0.1 µg/mL) Immunohistochemistry(5-15 µg/mL) |
目标/特异性 | Detects rat Growth Hormone in Western blots. In this format, approximately 10% cross-reactivity with recombinant human Growth Hormone is observed. |
使用方法 | Western Blot: 0.1 µg/mL Immunohistochemistry: 5-15 µg/mL |
来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
产品组分 |
供应商 | R&D Systems |
Entrez Gene IDs | 2688 (Human) |
纯化方式 | Antigen Affinity-purified |
免疫原 | E. coli-derived recombinant rat Growth Hormone Leu27-Phe216 Accession # P01244 |
生物活性 | Mouse, Rat |
标记 | Biotin |
溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
背景 | Growth Hormone, also named somatotropin, belongs to thesomatotropin/prolactin superfamily. It is synthesized in the anteriorpituitary gland. Growth Hormone regulates body growth by stimulatingthe secretion of IGF-I by liver and other tissues. |
运输条件 | Blue Ice |
存放说明 | -20℃ |
参考文献 |
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Growth Hormone in Rat Pituitary. Growth Hormone was detected in perfusion fixed frozen sections of rat pituitary using Goat Anti-Rat Growth Hormone Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF1566) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections. | |
Growth Hormone in Mouse Embryo. Growth Hormone was detected in immersion fixed frozen sections of mouse embryo using Goat Anti-Rat Growth Hormone Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF1566) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections. |