| 货号 | BAF935 |
| 别名 | ADAM 15; ADAM metallopeptidase domain 15; disintegrin-like, and cysteine-rich protein 15; EC 3.4.24; MDC15; MDC-15; Metargidin | 全称 | A Disintegrin and Metalloprotease-like Domain 15 |
| 反应种属 | Human |
| 应用 | Western Blot(0.1 µg/mL) Immunohistochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects human ADAM15 in Western blots. In Western blots, approximately 5% cross‑reactivity with recombinant mouse (rm) ADAM15 is observed and less than 1% cross‑reactivity with rmADAM9 and recombinant human ADAM8 is observed. |
| 使用方法 | Western Blot: 0.1 µg/mL Immunohistochemistry: 5-15 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 8751 (Human); 11490 (Mouse) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | S. frugiperda insect ovarian cell line Sf 21-derived recombinant human ADAM15 Met1-Ser693 Accession # Q13444 |
| 生物活性 | Human |
| 标记 | Biotin |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Members of the ADAM or MDC (Metalloprotease, Disintegrin, Cysteine-rich) family contain pro, metalloprotease-like, disintegrin-like, cysteine-rich, transmembrane and cytoplasmic domains. They play a fundamental role in diverse processes such as asthma, development, angiogenesis and cancer through their activities in cell adhesion/fusion, membrane protein shedding, and signal transduction. Over 30 members have been identified and about half of them are active metalloproteases such as ADAM8, 9, 10, 12 and 17/TACE. |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
| 参考文献 |
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ADAM15 in Human Prostate. ADAM15 was detected in immersion fixed paraffin-embedded sections of human prostate array using Goat Anti-Human ADAM15 Ectodomain Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF935) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections. |
