| 货号 | BAF8221 |
| 别名 | API3X-linked inhibitor of apoptosis protein; apoptosis inhibitor 3; baculoviral IAP repeat-containing 4; baculoviral IAP repeat-containing protein 4; BIRC4; BIRC4XLP2; E3 ubiquitin-protein ligase XIAP; EC 6.3.2.-; hIAP3; hIAP-3; hILP; IAP3; IAP-3; IAP-like protein; ILP; ILP1; Inhibitor of apoptosis protein 3; MIHA; X-linked IAP; X-linked inhibitor of apoptosis | 全称 | X-linked Inhibitor of Apoptosis |
| 反应种属 | Human/Mouse |
| 应用 | Western Blot(0.25 µg/mL) |
| 目标/特异性 | Detects human and mouse XIAP in Western blots. |
| 使用方法 | Western Blot: 0.25 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 331 (Human); 11798 (Mouse); 63879 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human XIAP Met1-Ser497 (Ser162Cys) Accession # P98170 |
| 生物活性 | Human, Mouse |
| 标记 | Biotin |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | XIAP (X-chromosome linked inhibitor of apoptosis) is a member of the apoptosis (IAP) family of proteins that inhibit caspases. The BIR2 domain of XIAP inhibits caspase-3 and caspase-7. The ability of XIAP to inhibit caspases is prevented by SMAC/Diablo through binding to XIAP-BIR2 and -BIR3 domains. |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
| 参考文献 |
|
Detection of Human and Mouse XIAP by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and C2C12 mouse myoblast cell line. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Human XIAP Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF8221) followed by Streptavidin-HRP (Catalog # DY998). A specific band was detected for XIAP at approximately 56 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2. |
