| 货号 | BAF5788 |
| 别名 | CAV; CAV2; caveolae protein, 20-kD; caveolin 2 isoform a and b; caveolin 2; caveolin-2; MGC12294 |
| 反应种属 | Human/Mouse/Rat |
| 应用 | Immunocytochemistry(5-15 µg/mL) |
| 目标/特异性 | Caveolin-2 antibodies are ideal for immunocytochemistry colocalization studies in caveolae. The unconjugated antibody detects endogenous human, mouse, and rat Caveolin-2 in Western blots. |
| 使用方法 | Immunocytochemistry: 5-15 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 858 (Human); 12390 (Mouse); 363425 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human Caveolin-2 Met1-Lys86 Accession # P51636 |
| 生物活性 | Human, Mouse, Rat |
| 标记 | Biotin |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Caveolin-2 is a cell membrane-associated member of the caveolin family of proteins. Although its predicted MW is 17 kDa, it runs anomalously at 21‑22 kDa on SDS‑PAGE. Caveolin-2 is not ubiquitous, but it is found in divergent cell types such as smooth muscle, endothelial, and skeletal muscle cells, plus fibroblasts and adipocytes. When expressed alone, Caveolin-2 forms monomers and homodimers; when expressed with Caveolin-1, it exists as part of a 150 kDa, Caveolin-1 associated oligomeric complex. Human Caveolin-2 is 162 amino acids (aa) in length. It contains a central membrane-embedded sequence (aa 87‑107) flanked by two cytoplasmic domains (aa 1‑86 and 108‑162). Caveolin-2 has been found phosphorylated at tyrosines 19 and 27, and at serines 20, 23 and 36. |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
| 参考文献 |
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Caveolin‑2 in HeLa Human Cell Line. Caveolin‑2 was detected in formaldehyde fixed HeLa human cervical epithelial carcinoma cell line using Goat Anti-Human/Mouse/Rat Caveolin‑2 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF5788) at 5 µg/mL overnight at 4° C. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (red; Catalog # NL999) and counterstained with DAPI (blue). Specific staining was localized to caveolae. View our protocol for Fluorescent ICC Staining of Cells on Coverslips. |
