| 货号 | BAF4634 |
| 别名 | BFPP; EGF-TM7-like; G protein-coupled receptor 56,7-transmembrane protein with no EGF-like N-terminal domains-1; Protein TM7XN1; TM7LN4; TM7LN4G-protein coupled receptor 56; TM7XN1; TM7XN1DKFZp781L1398 | 全称 | G Protein-coupled Receptor 56 |
| 反应种属 | Human |
| 应用 | Western Blot(0.1 µg/mL) |
| 目标/特异性 | Detects human GPR56 in Western blots. In Western blots, less than 1% cross-reactivity with recombinant human (rh) GPR30, rhGPR114, rhGPR115, rhGPR124, and rhGPR125 is observed. |
| 使用方法 | Western Blot: 0.1 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 9289 (Human); 14766 (Mouse); 260326 (Rat) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. The N Terminus of the Adhesion G Protein-coupled Receptor GPR56 Controls Receptor Signaling Activity. | |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Chinese hamster ovary cell line CHO-derived recombinant human GPR56 Arg26-Val342 Accession # AAP35975 |
| 生物活性 | Human |
| 标记 | Biotin |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | GPR56 is a member of the LN-TM7 family of adhesion-type 7-transmembrane (TM) G-protein coupled receptors (GPCR) with long extracellular N-termini (1‑3). The 693 amino acid (aa) human GPR56 contains a 25 aa signal sequence, a 377 aa N-terminal extracellular domain (ECD) and seven TM regions separated by short intracellular and extracellular regions. Like other LN-TM7 members, the ECD contains a highly glycosylated mucin-like stalk followed by a GPCR proteolytic cleavage site (GPS) (1, 4). Cleavage of the 60 kDa N-terminus from the 80 kDa full length form is needed for efficient cell surface expression (5, 6). While the cleaved portion may remain non-covalently associated, it has also been found in conditioned medium of cultured cells (5). Human GPR56 shares 71%, 72%, 80%, 80% and 79% aa identity with mouse, rat, canine, equine, and bovine GPR56 within the cleaved ECD. A functional splice variant lacking the GPS site and a non-functional splice variant lacking portions of the TM domains have also been described (4). A human brain developmental disorder, bilateral frontoparietal polymicrogyria, is associated with GPR56 mutations that also show impaired GPS cleavage, intracellular trafficking, and expression at the cell surface (5). GPR56 is widely distributed, with highest mRNA or expressed sequence tag expression in brain, thyroid, skin and female reproductive system (3, 4). GPR56 expression is upregulated during cell transformation and is high in melanomas, glioblastomas and astrocytomas, but downregulated in melanomas with high metastatic potential (2, 6‑8). Although the function of GPR56 is not completely known, it is clearly an adhesion protein (6‑8). Tissue transglutaminase (TG2) is one reported ligand, binding of which inhibits melanoma growth and metastasis (6). Association of GPR56 with the tetraspanin CD81 stabilizes its complex with Gaq/11 for cell signaling (9). |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
| 参考文献 |
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