Human FAP Biotinylated Affinity Purified PAb (50 UG)【科瑞奥博】

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Human FAP Biotinylated Affinity Purified PAb (50 UG)

货号： BAF3715 基本售价： 5532.3 元规格： -

产品信息

概述
货号	BAF3715
描述	For ELISA the Antibody Pairs information：Capture antibody：MAB3715-500；Detection antibody：BAF3715; and protein: 3715-SE-010
别名	170 kDa melanoma membrane-bound gelatinase; DKFZp686G13158; DPPIV; EC 3.4.21.-; FAP; FAPA; Fibroblast activation protein alpha; fibroblast activation protein, alpha; Integral membrane serine protease; Seprase
反应种属	Human
应用	Western Blot(0.5 µg/mL) ELISA Capture (Matched Antibody Pair)(2-8 µg/mL ) ELISA Detection (Matched Antibody Pair)(0.1-0.4 µg/mL ) ELISA Standard ( )
目标/特异性	Detects human Fibroblast Activation Protein alpha /FAP in ELISAs and Western blots. In sandwich immunoassays, less than 0.2% cross-reactivity with recombinant human DPPIV is observed.
使用方法	Western Blot: 0.5 µg/mL ELISA Capture (Matched Antibody Pair): 2-8 µg/mL ELISA Detection (Matched Antibody Pair): 0.1-0.4 µg/mL ELISA Standard :
来源	Reconstitute at 0.2 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	2191 (Human); 14089 (Mouse)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Cancer associated fibroblasts in pancreatic cancer are reprogrammed by tumor-induced alterations in genomic DNA methylation Cancer Res, 2016;0(0):. Species: Human Sample Type: Whole Cells Application: cell separation
纯化方式	Antigen Affinity-purified
免疫原	S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Fibroblast Activation Protein alpha /FAP Leu26-Asp760 Accession # Q12884
生物活性	Human
标记	Biotin
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	FAP (also known as seprase) is a Type II transmembrane serine protease that is structurally related to dipeptidyl peptidase IV (1). FAP has substrate specificity similar to dipeptidyl peptidase IV, which is specific for N-terminal Xaa-Pro sequences, but FAP is also an endopeptidase able to degrade gelatin and Type I collagen (2). The enzymatically active form of FAP is a dimer (3). FAP has a restricted tissue distribution. It is not detectable in normal tissues or resting fibroblasts, but is highly expressed on reactive stromal fibroblasts in epithelial cancers (4), in granulation tissue during wound healing, and in bone and soft tissue sarcomas (5). Because of its expression patterns and enzymatic activities, FAP is believed to play roles in tumor invasion, tissue remodeling, and wound repair.
运输条件	Blue Ice
存放说明	-20℃
参考文献	Scanlan, M.J. et al. (1994) Proc. Natl. Acad. Sci. USA 91:5657. Park, J.E. et al. (1999) J. Biol. Chem. 274:36505. Pineiro-Sanchez, M.L. et al. (1997) J. Biol. Chem. 272:7595. Garin-Chesa, P. et al. (1990) Proc. Natl. Acad. Sci. USA 87:7235. Rettig, W.J. et al. (1988) Proc. Natl. Acad. Sci. USA 85:3110.

参考图片

Detection of Human Fibroblast Activation Protein alpha /FAP by Western Blot. Western blot shows lysates of WI‑38 human lung fibroblast cell line. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Human Fibroblast Activation Protein alpha /FAP Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF3715) followed by Streptavidin-HRP (Catalog # DY998). A specific band was detected for Fibroblast Activation Protein alpha /FAP at approximately 97 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.