| 货号 | BAF3674 |
| 别名 | developmental pluripotency associated 4; developmental pluripotency-associated protein 4; FLJ10713,2410091M23Rik | 全称 | Developmental Pluripotency Associated 4 |
| 反应种属 | Human |
| 应用 | Western Blot(0.1 µg/mL) Intracellular Staining by Flow Cytometry(2.5 µg/106cells) |
| 目标/特异性 | Detects human DPPA4 in Western blots. In Western blots, approximately 10% cross-reactivity with recombinant mouse DPPA4 is observed and less than 1% cross-reactivity with recombinant human DPPA5 is observed. |
| 使用方法 | Western Blot: 0.1 µg/mL Intracellular Staining by Flow Cytometry: 2.5 µg/106cells |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 55211 (Human); 73693 (Mouse) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human DPPA4 Met1-Glu304 Accession # Q7L190 |
| 生物活性 | Human |
| 标记 | Biotin |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | DPPA molecules are a group of five name-only related proteins that are best described as a set of Oct4-related genes. They serve as markers for early embryonic and germline pluripotent cells. DPPA1 is transmembrane, DPPA2-4 contain DNA-binding domains, while DPPA5/ESG1 is a K homology RNA-binding (KH) domain containing protein that is expressed in embryonic germ cells and embryonic stem cells. DPPA5 is a potential marker for pluripotent stem cells. |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
| 参考文献 |
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Detection of DPPA4 in NTera‑2 Human Cell Line by Flow Cytometry. NTera‑2 human testicular embryonic carcinoma cell line was stained with Sheep Anti-Human DPPA4 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF3674, filled histogram) or isotype control antibody ???, followed by Streptavidin-Phycoerythrin (Catalog # F0040). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin. |
