| 货号 | BAF3646 |
| 别名 | bet; delta and Notch-like epidermal growth factor-related receptor; delta/notch-like EGF repeat containing; delta-notch-like EGF repeat-containing transmembrane; H_NH0150O02.1; UNQ26; WUGSC:H_NH0150O02.1 | 全称 | Delta/Notch-like EGF Repeat-containing Transmembrane |
| 反应种属 | Human |
| 应用 | Western Blot(0.2 µg/mL) Immunohistochemistry(5-15 µg/mL) Immunocytochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects human DNER in Western blots. In Western blots, approximately 30% cross-reactivity with recombinant mouse DNER is observed. |
| 使用方法 | Western Blot: 0.2 µg/mL Immunohistochemistry: 5-15 µg/mL Immunocytochemistry: 5-15 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 92737 (Human); 227325 (Mouse) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Biological and molecular heterogeneity of breast cancers correlates with their cancer stem cell content. | |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant human DNER Arg29-His637 Accession # Q8NFT8 |
| 生物活性 | Human |
| 标记 | Biotin |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | DNER (delta and notch-like EGF-related receptor; also BET) is a 120-150 kDa type I transmembrane glycoprotein that is reminescent of notch ligands such as Delta and Jagged. It is found on neuron dendrites and cell bodies but not on axons. Human DNER is 703 amino acids (aa) in length, and contains a 606 aa extracellular domain (ECD) (aa 35-640) plus a 76 aa cytoplasmic region. The ECD contains nine consecutive EGF-like domains, followed by one follistatin-like domain and a tenth EGF-like domain. DNER binding to notch-1 on glia induces morphological differentiation. Human DNER ECD is 89% aa identical to mouse DNER ECD. |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
| 参考文献 |
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Detection of Human DNER by Western Blot. Western blot shows lysates of human brain (cerebellum) tissue. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Human DNER Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF3646) followed by streptavidin-HRP. A specific band was detected for DNER at approximately 140 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
