| 货号 | BAF3548 |
| 别名 | Fasciclin I-like; MGC119510; MGC119511; OSF-2; OSF-2osteoblast specific factor 2 (fasciclin I-like); OSF2periodontal ligament-specific periostin; Osteoblast-specific factor 2; PDLPOSTN; periostin isoform thy2; periostin isoform thy4; periostin isoform thy6; periostin isoform thy8; periostin; periostin, osteoblast specific factor; PNRP11-412K4.1; POSTN; TRIF52 | 全称 | Osteoblast Specific Factor 2 |
| 反应种属 | Human |
| 应用 | Western Blot(0.1 µg/mL) |
| 目标/特异性 | Detects human Periostin/OSF-2 in Western blots. In this format, approximately 45% cross-reactivity with recombinant mouse Periostin/OSF-2 is observed. |
| 使用方法 | Western Blot: 0.1 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 10631 (Human); 50706 (Mouse); 361945 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant human Periostin/OSF-2 (R&D Systems, Catalog # 3548-F2) Ala22-Gln836 Accession # Q15063 |
| 生物活性 | Human |
| 标记 | Biotin |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Human OSF-2 (osteoblast-specific factor 2; also periostin) is a 170 kDa secreted homodimeric protein that belongs to the periostin family of the FAS1 superfamily of molecules (1-4). It is a TGF-beta inducible molecule that serves as both an adhesion molecule and tumor suppressor (2, 5, 6, 7). It is synthesized as an 836 amino acid (aa) precursor that contains a 21 aa signal sequence and an 815 aa mature region (2, 8). It is unknown if the molecule has any significant glycosylation (2). The human homodimer is not disulfide-linked (3). The molecule consists of two distinct regions. The N-terminus contains an 55 aa EMI domain, while the C-terminus contains four 130 aa fasciculin type 1 (FAS1) domains. The EMI domain is cysteine-rich and shows a highly basic alpha -helix (9). Each FAS1 repeat exhibits a novel seven-stranded beta ‑wedge with a multiple alpha -helical fold (1, 8). Three alternate splice forms are known that are C-terminal to the fourfold FAS1 repeats. These mature molecules are 758 and 761 aa in length. The first shows a one aa substitution for aa 649-706 of the mature molecule. The second shows a one aa substitution for aa 649-676, and a deletion of 27 aa between aa 784-810 of the mature molecule (10). The significance of the alternate splice forms is not clear. OSF-2 is known to bind to alpha vbeta3 and alpha vbeta5 integrins (3). It is synthesized by smooth muscle cells, fibroblasts, osteoblasts, and multiple carcinoma cell types (2, 3, 5, 7, 11, 12). OSF-2 induces expression of VEGFR2/KDR on endothelial cells (EC) by binding to EC alpha vbeta3(12). It also promotes cell transformation to a tumorigenic phenotype, accompanied by MMP-9 and fibronectin production and cell migration (13). Mature human OSF-2 is 91%, 96% and 91% aa identical to rat, dog, and mouse OSF-2, respectively. |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
| 参考文献 |
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