| 货号 | BAF2906 |
| 别名 | CSPB; CTLA-1; CTSGL1; Fragmentin-2; Granzyme-2; GRB; GrzB; GZMB; HLP; SECT |
| 反应种属 | Human |
| 应用 | Western Blot(0.1 µg/mL) Intracellular Staining by Flow Cytometry(2.5 µg/106cells) |
| 目标/特异性 | Detects human Granzyme B in Western blots. In Western blots, approximately 25% cross-reactivity with recombinant mouse (rm) Granzyme B and rmGranzyme D is observed. |
| 使用方法 | Western Blot: 0.1 µg/mL Intracellular Staining by Flow Cytometry: 2.5 µg/106cells |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 3002 (Human); 14939 (Mouse) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. HLA-E-restricted regulatory CD8(+) T cells are involved in development and control of human autoimmune type 1 diabetes. | |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant human Granzyme B Gly19-Tyr247 Accession # P10144 |
| 生物活性 | Human |
| 标记 | Biotin |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Granzyme B is a member of the granzyme family of the serine proteases found specifically in the cytotoxic granules of cytotoxic T lymphocytes (CTL) and natural killer (NK) cells (1, 2). Granzyme B plays an essential role in granule-mediated apoptosis and may have additional roles in rheumatoid arthritis and in bacterial and viral infections (3). It activates various caspases and cleaves proteins such as aggrecan (3). Human Granzyme B is synthesized as a precursor (247 residues) with a signal peptide (residues 1‑18), a pro peptide (residues 19‑20), and a mature chain (residues 21‑247) (4‑6). The rhGranzyme B consisting of residues 19‑247 was expressed and purified. After being activated by active cathepsin C, rhGranzyme B cleaves a thioester substrate described previously (3). |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
| 参考文献 |
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