| 货号 | BAF2739 |
| 别名 | BF; B-factor, properdin; BFAHUS4; BFD; C3 proaccelerator; C3 proactivator; C3/C5 convertase; CFAB; CFB; complement factor B; EC 3.4.21; EC 3.4.21.47; FB; FBI12; GBG; GBGCFAB; Glycine-rich beta glycoprotein; glycine-rich beta-glycoprotein; H2-Bf; PBF2; Properdin factor B |
| 反应种属 | Human |
| 应用 | Western Blot(0.1 µg/mL) |
| 目标/特异性 | Detects human Complement Factor B in Western blots. In this format, less than 2% cross-reactivity with recombinant human (rh) Factor D and rhFactor I is observed. |
| 使用方法 | Western Blot: 0.1 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 629 (Human); 14962 (Mouse); 294257 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant human Complement Factor B Thr26-Leu764 with a Gln34Arg substitution Accession # AAA16820 |
| 生物活性 | Human |
| 标记 | Biotin |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Complement Factor B (also C3/C5 convertase, GBC and PBF2) is a 93-95 kDa glycoprotein member of the peptidase S1 family of enzymes. It is expressed by hepatocytes and macrophages, and serves as a substrate for complement factor D. Following binding of Factor B to membrane-bound C3b, Factor D acts on Factor B to generate a 33 kDa N-terminal fragment (Ba), plus a 60 kDa C-terminal fragment (Bb) that remains associated with C3b to createe a serine protease. Mature human Factor B is 739 amino acids (aa) in length (aa 26-764). It contains three Sushi domains (aa 35-160), one vWFA domain (aa 270-469), and a large peptidase S1 region (aa 477-757). Cleavage between Arg259Lys260 generates the Ba chain (aa #26-259) and the Bb chain (aa 260-764). There are two potential isoform variants. One shows a premature truncation after Gly589, while another shows a 79 aa substitution for aa 543-764. Over aa 26-764, human shares 85% aa identity with mouse Factor B. |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
| 参考文献 |
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