Human GATA-4 Biotinylated Affinity Purified PAb (50 UG)【科瑞奥博】

产品中心

当前位置：首页>产品中心

Human GATA-4 Biotinylated Affinity Purified PAb (50 UG)

货号： BAF2606 基本售价： 5532.3 元规格： -

产品信息

概述
货号	BAF2606
别名	GATA binding protein 4; GATA-binding factor 4; GATA-binding protein 4; MGC126629; transcription factor GATA-4
反应种属	Human
应用	Western Blot(0.1 µg/mL) Immunocytochemistry(5-15 µg/mL)
目标/特异性	Detects human GATA‑4 in Western blots. In Western blots, less than 2% cross-reactivity with recombinant human GATA-1, -2, -3, -5, and -6 is observed.
使用方法	Western Blot: 0.1 µg/mL Immunocytochemistry: 5-15 µg/mL
来源	Reconstitute at 0.2 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	2626 (Human)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Isolation of primitive endoderm, mesoderm, vascular endothelial and trophoblast progenitors from human pluripotent stem cells. Authors: Drukker M, Tang C, Ardehali R Nat. Biotechnol., 2012;30(6):531-42. Species: Human Sample Type: Whole Cells Application: ICC
纯化方式	Antigen Affinity-purified
免疫原	E. coli-derived recombinant human GATA-4 Met27-Phe211 Accession # P43694
生物活性	Human
标记	Biotin
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	GATA-4 belongs to the GATA family of transcription factors, which bindto the consensus DNA sequence (A/T) GATA (A/G) to control diversetissue-specific programs of gene expression and morphogenesis. It iswidely expressed in mesodermal- and endodermal-derived tissues. GATA-4interacts with other transcription factors such as NKX2.5, and myocyteenhancer factor 2 to regulate cardiac myocyte-specific gene expression.Human GATA-4 share 93%, 93% and 92% amino acid sequence identity withporcine, rat and mouse GATA-4, respectively.
运输条件	Blue Ice
存放说明	-20℃
参考文献	Cardiac Stem Cell Markers Definitive Endoderm Markers Early Endodermal Lineage Markers GATA Transcription Factors Hepatic Endoderm Hepatic Endoderm Cell Markers Human Pluripotent Stem Cell Antibody Array ICC / IHC Images: GATA-4 Mesenchymal Stem Cell Transcription Factors Primitive Endoderm Markers Transcription Factors in the Akt Pathway

参考图片

GATA‑4 in iBJ6 iPS Cell Line. GATA‑4 was detected in immersion fixed iBJ6, an induced pluripotent stem cell line, differentiated to endoderm using Goat Anti-Human GATA‑4 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF2606) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (red; Catalog # NL999) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.