| 货号 | BAF2570 |
| 描述 | For ELISA the Antibody Pairs information：Capture antibody：MAB2570-500；Detection antibody：BAF2570; and protein: 2570-ND-050 |
| 别名 | enactin; Entactin; Entactin-1; NID1; NID-1; NIDentactin; nidogen (enactin); nidogen 1; nidogen-1 |
| 反应种属 | Human |
| 应用 | Western Blot,Immunohistochemistry,ELISA Capture (Matched Antibody Pair),ELISA Detection (Matched Antibody Pair),ELISA Standard |
| 目标/特异性 | Detects human Nidogen‑1/Entactin in ELISAs and Western blots. In sandwich immunoassays, less than 0.2% cross‑reactivity with recombinant human Nidogen-2 is observed. |
| 使用方法 | Western Blot: 0.1 µg/mL Immunohistochemistry: 5-15 µg/mL ELISA Capture (Matched Antibody Pair): 2-8 µg/mL ELISA Detection (Matched Antibody Pair): 0.1-0.4 µg/mL ELISA Standard : |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 4811 (Human) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant human Nidogen‑1/Entactin Leu29-Lys1114 (Gln1113Arg) Accession # AAH45606.1 |
| 生物活性 | Human |
| 标记 | Biotin |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Nidogen-2 (also called entactin-2) is a 200 kDa, secreted, basement membrane glycoprotein. Nidogens-1 and -2 are expressed in nearly all basement membranes where they interact with laminins, collagen type IV, and proteoglycan family members. Nidogen-2 contains three globular domains (G1-3) separated by a link region and an extended rod-shaped segment. The G1 domain is reported to bind type IV collagen, the G2 domain interacts with perlecan, and C-terminal G3 is associated with laminin binding. There is one EGF-like motif and a short peptide that ligates alpha 3 beta 1 integrins. The rod-shaped segment contains four additional EGF-like motifs and two thyroglobulin type 1 domains that serve as a binding site for alpha V beta 3 integrins. |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
| 参考文献 |
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Nidogen‑1/Entactin in Human Heart. Nidogen‑1/Entactin was detected in immersion fixed paraffin-embedded sections of human heart using Goat Anti-Human Nidogen‑1/Entactin Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF2570) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections. |
