| 货号 | BAF253 |
| 别名 | CD125 antigen; CD125; CDw125CD125; HSIL5R3; IL-5 receptor subunit alpha; IL-5R subunit alpha; IL5R; IL5RA; IL-5RA; IL-5R-alpha; interleukin 5 receptor type 3; interleukin 5 receptor, alpha; interleukin-5 receptor alpha chain; interleukin-5 receptor alpha subunit; interleukin-5 receptor subunit alpha; MGC26560 | 全称 | Interleukin 5 Receptor alpha |
| 反应种属 | Human |
| 应用 | Western Blot(0.1 µg/mL) |
| 目标/特异性 | Detects human IL‑5 R alpha /CD125 in Western blots. In this format, less than 5% cross-reactivity with recombinant human (rh) IL‑2 sR beta, rhIL‑2 sR gamma, rhIL-3 sR, rhIL-4 sR, rhIL-1 sRII, rhIL-6 sR, rhIL-9 sR, and rhIL-10 sR is observed. |
| 使用方法 | Western Blot: 0.1 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 3568 (Human); 16192 (Mouse) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Rapid chemokine secretion from endothelial cells originates from 2 distinct compartments. | |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | S. frugiperda insect ovarian cell line Sf 21-derived recombinant human IL‑5 R alpha /CD125 |
| 生物活性 | Human |
| 标记 | Biotin |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Interleukin 5, produced primarily by activated T cells and mast cells, has diverse biological effects on a variety of cell types. Human IL-5 is a potent eosinophil differentiation and activation factor in vivoandin vitro. Additionally, it has also been reported that IL-5 can stimulate the proliferation and/or differentiation of basophils and B cells. The multiple effects of IL-5 are mediated by binding of the cytokine to specific cell surface receptors expressed on target cells. As is the case with many other cytokines, the functional high-affinity receptor for IL-5 is a complex consisting of a ligand binding subunit ( alpha chain) and a second subunit ( beta chain) that can modulate the ligand binding affinity of the receptor complex. In the case of IL-5, the beta subunit is shared with the high affinity receptor complexes for IL-3 and GM-CSF. The beta chain does not bind any of the cytokines in question but is indispensable for the cytokine-mediated signaling. cDNA clones for the alpha chain (IL-5 R alpha ) of both the mouse and human high affinity IL-5 receptor complexes have been isolated. Human and mouse IL-5 R alpha are both members of the hematopoietin receptor superfamily characterized by the presence of the WSXWS, and a four cysteine residue motif in the extracellular domain of the transmembrane protein. In addition to the cDNA clone encoding the full-length transmembrane protein, cDNA clones that arise from alternative splicing and that encode soluble secreted forms of IL-5 R alpha have been isolated from mouse as well as human cells. A naturally-occurring soluble form of the IL-5 R alpha has been detected in biological fluids of autoimmune-prone mice and mice bearing chronic B cell leukemia (BCL1). A recombinant human IL-5 soluble receptor alpha has been shown to bind the human IL-5 dimer in a 1:1 ratio and acts as a human IL-5 antagonist. This molecule inhibits the proliferation of IL-5-dependent cell lines and blocks human umbilical cord blood eosinophil differentiation. |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
| 参考文献 |
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