| 货号 | BAF2444 |
| 别名 | cardiac-specific homeo box; cardiac-specific homeobox 1; Cardiac-specific homeobox; CHNG5; CSX1; CSXFLJ97195; FLJ52202; FLJ99536; Homeobox protein CSX; Homeobox protein NK-2 homolog E; homeobox protein Nkx-2.5; NK2 transcription factor related, locus 5 (Drosophila); NKX2.5FLJ97166; NKX2-5; NKX2E; NKX2EFLJ97197; NKX4-1; tinman paralog | 全称 | NK2 Homeobox 5 |
| 反应种属 | Human |
| 应用 | Western Blot(0.1 µg/mL) Immunohistochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects human NKX2.5 in Western blots. |
| 使用方法 | Western Blot: 0.1 µg/mL Immunohistochemistry: 5-15 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 1482 (Human) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human NKX2.5 Gln24-Arg137 Accession # P52952 |
| 生物活性 | Human |
| 标记 | Biotin |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | The homeobox transcriptional factor NKX2.5 (Homeobox protein NK-2 homolog E) specifies cardiac and visceral mesoderm, and plays an essential role in heart development. |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
| 参考文献 |
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NKX2.5 in Human Heart. NKX2.5 was detected in immersion fixed paraffin-embedded sections of human heart using Goat Anti-Human NKX2.5 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF2444) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections. |
